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Abstract
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Article Information:
Temperature Adaptation Study on Probiotic Bacillus subtilis SK09 Based on its Extracellular Proteins
G. Sreekumar and K. Soundarajan
Corresponding Author: G. Sreekumar
Submitted: 2010 July, 01
Accepted: 2010 July, 27
Published: 2010 September, 25 |
Abstract:
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The assessment of the thermal adaptation characteristics of a probiotic organism is crucial since the
probiotic bacteria are sensitive to thermal stress created by the gut environment. Probiotic Bacillus subtilis SK09
was subjected to varying temperatures growth conditions and checked for its thermal adaptation. Experiments
were carried out with cultures grown in Nutrient broth at three different temperatures namely 25, 35 and 40ºC.
After 24 h the extracellular secretions present in the cell free supernatant were analyzed for amylase and
$-galactosidase activity as well the net protein content. Bacillus subtilis SK09 showed a prominent growth and
protein profile in 35ºC with maximum enzyme activity and total protein production compared to other two
conditions. At 40°C the strain exhibited a 30 kDa protein which might have been induced due to thermal stress.
Key words: Amylase, β - galactosidase, thermal adaptation, thermal stress, probiotic, ,
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Abstract
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Cite this Reference:
G. Sreekumar and K. Soundarajan, . Temperature Adaptation Study on Probiotic Bacillus subtilis SK09 Based on its Extracellular Proteins. Advance Journal of Food Science and Technology, (5): Page No: 246-249.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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