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    Abstract
2009 (Vol. 1, Issue: 2)
Article Information:

Pre-breeding Efforts to Utilize Wild Morus Species and DNA Fingerprinting of F1 Hybrids Using RAPD

T. Amalendu and K. Chandrakant Kamble
Corresponding Author:  AMALENDU TIKADER 

Key words:  Crossing behaviour, inter and intra-specific hybrids, molecular tools and m ulberry, , , ,
Vol. 1 , (2): Page No: 36-42
Submitted Accepted Published
2009 June, 18 2009 August, 05
Abstract:

Morus laevigata and Morus serrata are the wild Morus species of mulberry available in India possess unique features of bigger leaf size, higher leaf moisture and moisture retention, higher protein and carbohydrate with high adaptability to adverse climatic condition. In an effort to transfer these traits to cultivated species, inter-specific hybridization was effected between M. indica with M. laevigata and M. indica with M. serrata. After repeated trial of hybridization, successful F1 seeds were obtained among the crosses. The F1 hybrids showed better performance than female parent in most of the characters while it was better than m ale parent for few characters in M. indica with M. laevigata. In another cross, the F1 hybrids showed better performance than both the parents for most of the characters (M. indica x M. serrata). The crosses are expected to carry some genetic load, as the wild species were genetically and geographically distant and carry valuable genes. Polymorphism of genomic DNAs of nine parents and their seven hybrids were obtained from two wild and four cultivated species. Twelve arbitrary primers of Random Amplified Polymorphic DNA (RAPD) unraveled abundant polymorphism. Of the F1 hybrids, the banding pattern indicates similar like their respective parents. However, a few hybrids showed unique bands, which are different from respective parents. The RAPD technique demonstrated that hereditary variability occurred in between wild and cultivated Morus species at inter and intra-specific levels.
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  Cite this Reference:
T. Amalendu and K. Chandrakant Kamble, 2009. Pre-breeding Efforts to Utilize Wild Morus Species and DNA Fingerprinting of F1 Hybrids Using RAPD.  Asian Journal of Agricultural Sciences, 1(2): Page No: 36-42.
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ISSN (Online):  2041-3890
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