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2009 (Vol. 1, Issue: 2)
Article Information:

Callus Induction of Ocimum sanctum and Estimation of Its Total Flavonoids Content

Zi Xiong Lim, Anna Pick Kiong Ling and Sobri Hussein
Corresponding Author:  Anna Pick Kiong Ling 

Key words:  Anti-oxidant, essential oil, Holy Basil, Ocimum sanctum, orientin, vicenin,
Vol. 1 , (2): Page No: 55-61
Submitted Accepted Published
2009 July, 14 2009 July, 28

Ocimum sanctum, also known as “H oly Basil” is one of the most common medicinal plants used by diverse cultures and tribal groups. Callus induction from leaf explants of O. sanctum was conducted by incubating leaf explants on Murashige and Skoog (MS) medium supplemented with 2, 4!dichlorophenoxyacetic acid (2,4!D), picloram, and indole-butyric acid (IBA) at 0, 1, 3, and 5 mg/L as well as the combination of 3 mg/L picloram with different concentrations (0, 0.5, 1.0, 1.5, and 2.0 m g/L) of 6!benzylaminopurine (BAP) or kinetin. Results obtained from the studies revealed that all the leaf explants incubated on phytohormonesupplemented medium formed callus. Leaf explants grown on 3 mg/L picloram formed callus after 8±1 days of culture, and degree of callus formation w as found to be the highest (++++) among all the single auxin treatments. In contrast, the degree of callus formed from the leaf explants cultured on MS medium supplemented with combination of auxin and cytokinins were evidently lower than those in the single auxin treatments. Leaf explants cultured on kinetin-supplemented MS medium showed a higher degree of callus formation (++++) as compared to BAP-supplemented MS medium. There was no significant difference between the days of callus formation among all the cytokinin-supplemented treatments. The total flavonoids content of leaf-derived callus cultured on 3 mg/L picloram w ere also estimated and compared with the in vivo leaf tissues. The aluminium (III) chloride colorimetric assay revealed that the total flavonoids content of in vivo leaf tissues of O. sanctum were 2.2 times higher than leaf-derived callus, whereby the former produced 0.733±0.077 miligram catechin equivalent per gram fresh weight (mg CE/g fresh weight), while the latter only yielded 0.333±0.043 mg CE/g fresh weight.
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  Cite this Reference:
Zi Xiong Lim, Anna Pick Kiong Ling and Sobri Hussein, 2009. Callus Induction of Ocimum sanctum and Estimation of Its Total Flavonoids Content.  Asian Journal of Agricultural Sciences, 1(2): Page No: 55-61.
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ISSN (Online):  2041-3890
ISSN (Print):   2041-3882
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