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Abstract
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Article Information:
Production of Single Oospore Progeny (SOP) of Phytophtora infestans: Inheritance of Mating Type and Mycelial Morphotypes
Diana M. Earnshaw
Corresponding Author: Diana M. Earnshaw
Submitted: 2011 September, 29
Accepted: 2011 November, 04
Published: 2012 January, 25 |
Abstract:
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The aim of this study was to produce sexual progeny (oospores), of the causal agent of late blight,
Phytophthora infestans, study the inheritance of mating type and morphological appearances of these progeny.
This pseudofungus exists in two distinct mating types: A1 and A2 mainly in Mexico. However, reports indicate
presence of both mating types in many countries all over the world which indicates possibilities of sexual
reproduction. The experiment was conducted at the University of Wales, Bangor, in the School of Biological
Sciences. Three A1 parental isolates 96.89.43and 96.69 (both UK) and P9463 (California, USA) were mated
with a common A2 isolate 96.70 (UK). About 545 Single Oospore Progeny (SOP) were obtained. The A1:A2
ratio was 3:1 for cross #1; 1:1 for cross #2; and 3:1 for cross #3. All A1 SOPs and 7% of the A2 SOPs had nonlumpy
(-) morphology, the rest (93%) of the A2 SOPs exhibited lumpiness of varying degrees. Lumpiness
varied from slighty lumpy (+) (17%), lumpy (++) (56%), to being very lumpy (+++)(20%). No visible
sporangiophores observed among the very lumpy progeny. The A2 SOP were lumpy as opposed to non-lumpy
A2 parent, which was a fluffy isolate just like the A1 parents. Scanning electron microscope revealed that the
lumpiness was an aggregate of short-branching hyphae. Lumpiness of A2 isolates has not been reported in
nature but observed in laboratories. This could be due to the failure by such isolates to survive as in this study
such isolates were observed to produce very few sporangia.
Key words: Lumpiness, mating type, Phytophthora infestans, sexual progeny, single oospore progeny, sporangiospore,
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Cite this Reference:
Diana M. Earnshaw, . Production of Single Oospore Progeny (SOP) of Phytophtora infestans: Inheritance of Mating Type and Mycelial Morphotypes. Asian Journal of Agricultural Sciences, (1): 43-52.
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ISSN (Online): 2041-3890
ISSN (Print): 2041-3882 |
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