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2012 (Vol. 4, Issue: 4)
Article Information:

Resolving Genetic Relationships Among Aegilops L. and Triticum L. Species Using Analysis of Chloroplast DNA by Cleaved Amplified Polymorphic Sequence (CAPS)

Lina Mammdouh Alnaddaf, Mohammad Yahia Moualla and Nadia Haider
Corresponding Author:  lina mammdouh alnaddaf 

Key words:  Aegilops, CAPS, chloroplast, DNA, genetic relationships, Triticum,
Vol. 4 , (4): 270-279
Submitted Accepted Published
April 17, 2012 May 10, 2012 July 15, 2012
Abstract:

Studying of genetic relationships among Triticum L. and Aegilops L. species by using Cleaved Amplified Polymorphic Sequence (CAPS) to investigate the polymorphism in chloroplast DNA (cpDNA) among 20 Aegilops L. and 7 Triticum L. species and one accession of Amblyopyrum muticum. 23 chloroplast regions were amplified then Restriction digests were performed on PCR amplicons instructions using 72 singlecut Endonucleases. The rseults indicate that there are 16 accessions from Triticum and Aegilops species have positive and unique restriction profiles in 5 loci. Inaddition 11 accessions from Aegilops and one accession of Amblyopyrum muticum have identical restriction profiles in all loci.
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  Cite this Reference:
Lina Mammdouh Alnaddaf, Mohammad Yahia Moualla and Nadia Haider, 2012. Resolving Genetic Relationships Among Aegilops L. and Triticum L. Species Using Analysis of Chloroplast DNA by Cleaved Amplified Polymorphic Sequence (CAPS).  Asian Journal of Agricultural Sciences, 4(4): 270-279.
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ISSN (Online):  2041-3890
ISSN (Print):   2041-3882
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