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2013 (Vol. 5, Issue: 5)
Article Information:

Genetic Relationships among Aegilops L. and Triticum L. Species based on the Internal Transcribed Spacer Sequences of nrDNA (ITS)

Lina Mammdouh Alnaddaf, Mohammad Yahia Moualla and Nadia Haider
Corresponding Author:  Lina Mammdouh Alnaddaf 

Key words:  Aegilops, genetic relationships, internal transcribed spacer, ITS, Triticum, ,
Vol. 5 , (5): 108-117
Submitted Accepted Published
April 11, 2013 April 29, 2013 September 25, 2013
Abstract:

Studying of genetic relationships among Triticum L. and Aegilops L. L. species by direct sequencing of PCR-amplified Internal Transcribed Spacer (ITS) of nuclear ribosomal DNA to investigate the polymorphism in nucleotide sequences among 19 Aegilops L. and 7 Triticum L. species. ITS sequences were aligned with CLUSTAL W 2.1 multiple sequence aligment program. The phylogenetic relationships among species were reconstructed using Unweighted Pair Group Mean Arithmetic average (UPGMA) and Neighbor-Joining (NJ) methods. ITS region ranged from 600 to 602 bp. The length of ITS1 was 221-222 bp and ITS2 was 215-217 bp. The 5.8S subunit was 163 bp long. The G+C content of the ITS1 region ranged from (60.3 to 63.9) % in all Triticum and Aegilops species. The G+C content of the 5.8S subunit was entirely identical in all species, 59.5%. The G+C content of the ITS2 region ranged from 59.9 to 63.5%. There were 67 variable sites (11.12%) in the entire ITS region. The phylogenetic relationships among species were reconstructed using Unweighted Pair Group Mean Arithmetic average (UPGMA) were discussed.
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  Cite this Reference:
Lina Mammdouh Alnaddaf, Mohammad Yahia Moualla and Nadia Haider, 2013. Genetic Relationships among Aegilops L. and Triticum L. Species based on the Internal Transcribed Spacer Sequences of nrDNA (ITS).  Asian Journal of Agricultural Sciences, 5(5): 108-117.
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ISSN (Online):  2041-3890
ISSN (Print):   2041-3882
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