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Abstract
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Article Information:
Genetic Relationships among Aegilops L. and Triticum L. Species based on the Internal Transcribed Spacer Sequences of nrDNA (ITS)
Lina Mammdouh Alnaddaf, Mohammad Yahia Moualla and Nadia Haider
Corresponding Author: Lina Mammdouh Alnaddaf
Submitted: April 11, 2013
Accepted: April 29, 2013
Published: September 25, 2013 |
Abstract:
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Studying of genetic relationships among Triticum L. and Aegilops L. L. species by direct sequencing of PCR-amplified Internal Transcribed Spacer (ITS) of nuclear ribosomal DNA to investigate the polymorphism in nucleotide sequences among 19 Aegilops L. and 7 Triticum L. species. ITS sequences were aligned with CLUSTAL W 2.1 multiple sequence aligment program. The phylogenetic relationships among species were reconstructed using Unweighted Pair Group Mean Arithmetic average (UPGMA) and Neighbor-Joining (NJ) methods. ITS region ranged from 600 to 602 bp. The length of ITS1 was 221-222 bp and ITS2 was 215-217 bp. The 5.8S subunit was 163 bp long. The G+C content of the ITS1 region ranged from (60.3 to 63.9) % in all Triticum and Aegilops species. The G+C content of the 5.8S subunit was entirely identical in all species, 59.5%. The G+C content of the ITS2 region ranged from 59.9 to 63.5%. There were 67 variable sites (11.12%) in the entire ITS region. The phylogenetic relationships among species were reconstructed using Unweighted Pair Group Mean Arithmetic average (UPGMA) were discussed.
Key words: Aegilops, genetic relationships, internal transcribed spacer, ITS, Triticum, ,
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Cite this Reference:
Lina Mammdouh Alnaddaf, Mohammad Yahia Moualla and Nadia Haider, . Genetic Relationships among Aegilops L. and Triticum L. Species based on the Internal Transcribed Spacer Sequences of nrDNA (ITS). Asian Journal of Agricultural Sciences, (5): 108-117.
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ISSN (Online): 2041-3890
ISSN (Print): 2041-3882 |
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