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2010 (Vol. 2, Issue: 2)
Article Information:

Estimation of Genetic Diversity in Capsicum Germplasm Using Randomly Amplified Polymorphic DNA

Nazia Akbar, Habib Ahmad, Sajidul Ghafoor, Khaist Begum, Sahib Gul Afridi, Ikram Muhammad and Imtiaz Ahmed Khan
Corresponding Author:  Imtiaz Ahmed Khan 

Key words:  Capsicum annum, dendrogram, genetic distance, phylogenetic relationship, randomly amplified polymorphic DNA, ,
Vol. 2 , (2): Page No: 53-56
Submitted Accepted Published
2010 January, 22 2010 February, 01 2010 April, 10
Abstract:

Present study was undertaken to estimate genetics diversity in local and exotic genotypes of Capsicum (chili) using Randomly Amplified polymorphic DNA (RAPD) primers. Four RAPD primes were used to amplify genomic DNA from twenty five Capsicum genotypes. DNA fragments of various sizes ranging from 100bp to >1000bp were amplified in various Capsicum genotypes. On an average 3 alleles per genotype were amplified during present study. Estimates of Genetic distances (GD) ranged from 0 to 100%. Twentyfive genotypes were clustered in four groups A, B, C and D. It was concluded that genotypes Tomato bibber 1 and Turkish genotype B 1 were most distantly related to each other and hence it is recommended that these two genotypes should be crossed to create a segregating population w ith maximum genetic diversity.
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  Cite this Reference:
Nazia Akbar, Habib Ahmad, Sajidul Ghafoor, Khaist Begum, Sahib Gul Afridi, Ikram Muhammad and Imtiaz Ahmed Khan, 2010. Estimation of Genetic Diversity in Capsicum Germplasm Using Randomly Amplified Polymorphic DNA.  Asian Journal of Agricultural Sciences, 2(2): Page No: 53-56.
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ISSN (Online):  2041-3890
ISSN (Print):   2041-3882
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