During present work, comparative Proteomics were employed for elaborating genetic diversity in 27 accessions of 6 genera of family Papilionaceae (legumes family). Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) was employed for analyzing seed storage protein of the species. The Proteomic assay comprised a total of 296 reliably score able protein alleles identified in the twenty-seven accessions of legumes. The genetic distance estimates ranged from 0-100%. Phylogenetic relationship among the legume based upon cluster analysis assorted all the accessions in to 4 groups A, B C and D comprising 15, 5, 4 and 3 accessions, respectively.