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2012 (Vol. 4, Issue: 6)
Article Information:

Characterization Analysis of Response of Alcohol Dehydrogenase Gene (ADH1) in Coix lacroyma>/em> jobi L. to Waterlogging Stress

Daqing-chen, Yunfeng-zhu, Gongqing-wu and Yanan-li
Corresponding Author:  Yanan-Li 

Key words:  Alcphol Dehydrogenase (ADH1), Coix lacroyma jobi L., expression analysis, gene cloning, , , ,
Vol. 4 , (6): 417-425
Submitted Accepted Published
September 11, 2012 October 19, 2012 December 20, 2012
Abstract:

The aim of this study was focused on response of Alcohol Dehydrogenase gene (ADH1) in Coix to waterlogging stress. Based on the conserved sequence of Alcphol Dehydrogenase (ADH1) gene in maize, rice, and wheat, primers were designed to isolate theADH1 product. The full-length sequence of cDNA was firstly cloned by using RACE technology. The acquired gene contains an open reading frame (ORF, DQ455071.2) of 1140 bp and encodes 379 amino acids residues with the molecular weight and theoretical isoelectric of 40.965 and 6.13 KD, respectively. The BlastN/P analysis revealed that the sequence was highly homologous with gramineous plants such as ADH1 in maize, rice, and wheat. Moreover, it could be found in the phylogenetic tree that the origin of ADH1- encoding protein was most close to gramineous plants. It was predicted that it had at least two standard transmembrane segments, and the three-dimensional structure had 54.38% consistency with the reference model of 2 fzwa. The characteristic belt of ADH1 target protein was obtained by prokaryotic expression. Semi-quantitative analysis suggested thatADH1 gene expression was induced by waterlogging, and the expression in the root tip reached the highest level after 4 h of waterlogging, while ADH enzyme activity was also increased after waterlogging and reached the highest level after 6 h. Significant difference occurred in the ADH enzyme activities at different treatment time (p<0.05). Results indicated that ADH1 was sensitive to waterlogging and took part in tolerant and adaptive process under anaerobic environment.
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  Cite this Reference:
Daqing-chen, Yunfeng-zhu, Gongqing-wu and Yanan-li, 2012. Characterization Analysis of Response of Alcohol Dehydrogenase Gene (ADH1) in Coix lacroyma>/em> jobi L. to Waterlogging Stress.  Advance Journal of Food Science and Technology, 4(6): 417-425.
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