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    Abstract
2012 (Vol. 4, Issue: 6)
Article Information:

Investigation of Terahertz Spectral Signatures of DNA of Pine Wood Nematode

Ling Jiang, Chun Li, Lin Huang, Zhenwei Zhang, Cunlin Zhang and Yunfei Liu
Corresponding Author:  Ling Jiang 

Key words:  Absorption frequency, Bm nematode, Bx nematode, terahertz spectroscopy, , ,
Vol. 4 , (6): 426-429
Submitted Accepted Published
September 13, 2012 October 09, 2012 December 20, 2012
Abstract:

In this study, we present the fingerprint characteristic about the identification of harmful Bursaphelenchus xylophilus (Bx) nematode and harmless Bursaphelenchus mucronatus (Bm) nematode by applying terahertz spectroscopic techniques. We measure the transmission of the Deoxyribose Nucleic Acid (DNA) of the Bx and the Bm samples and their corresponding Polymerase Chain Reaction (PCR) amplification segments of the DNA molecules by the Terahertz Domain Spectroscopy (THz-TDS) and the Fourier Transform Infrared spectroscopy (FTIR). The low-frequency absorption features measured by THz-TDS exhibits different absorption peaks (i.e., 0.07 and 0.11 THz) for the Bx and Bm samples respectively. The calculated relative refractive index of the Bm-PCR segment is found to be much smaller compared with other three samples. The higher frequency characteristic of the four samples measured by the FTIR spectroscopy shows similar absorption peaks, except that the Bm-DNA provides a smooth differential feature of absorption at 5.46 THz. These measurement results indicate theBm samples including DNA and PCR segment indeed have unique signature behaviors different from the Bx samples. It demonstrates that the terahertz spectroscopic technique is a useful method to distinguish the Bx and the Bmís DNA and PCR segments by locating the absorption frequency.
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  Cite this Reference:
Ling Jiang, Chun Li, Lin Huang, Zhenwei Zhang, Cunlin Zhang and Yunfei Liu, 2012. Investigation of Terahertz Spectral Signatures of DNA of Pine Wood Nematode.  Advance Journal of Food Science and Technology, 4(6): 426-429.
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ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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