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Abstract
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Article Information:
Research on Extraction and Characterization of Cellulase from Commercial Enzyme Preparation
Guowei Shu, Hui Yang, He Chen, Zhiwei Yang
Corresponding Author: Guowei Shu
Submitted: February 15, 2013
Accepted: March 13, 2013
Published: July 05, 2013 |
Abstract:
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The extraction of cellulase from commercial enzyme preparation by ammonium sulfate precipitation and its enzymatic characterization were studied. The results were as follows: the conditions of precipitation was 60% saturation of ammonium sulfate, the recovery rate of carboxymethylcellulase (CMCase) and Filter Paper enzyme Activity (FPA) were 73.8 and 71.4%, respectively. The enzyme has optimal temperature of 55°C, optimal pH of 4.8, the ions of Co2+, Mn2+ in the buffer lowered the activity of CMCase, but the Cu2+ in low concentration activated the CMCase. The ions of Co2+, Mn2+ and Al2+ in the buffer lowered the activity of FPA, but the Cu2+ and K+ in low concentration activated the FPA; Km and Vmax of the enzyme were 2.22×10-3 g/mL and 1.11×104 U/h using Lineweaver-Burk graphic method, respectively.
Key words: Carboxymethylcellulase, cellulase, filter paper activity, enzymatic property, salting out, ,
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Cite this Reference:
Guowei Shu, Hui Yang, He Chen, Zhiwei Yang, . Research on Extraction and Characterization of Cellulase from Commercial Enzyme Preparation. Advance Journal of Food Science and Technology, (07): 839-842.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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