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Abstract
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Article Information:
Effect of Different Carbon Source on Expression of Carotenogenic Genes and Astaxanthin Production in Xanthophyllomyces dendrorhous
Wei Wu and Xin Yu
Corresponding Author: Wei Wu
Submitted: June 25, 2013
Accepted: July 10, 2013
Published: October 05, 2013 |
Abstract:
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The present research gives an insight into astaxanthin production, as well as transcription differences of four key carotenogenic genes, in Xanthophyllomyces dendrorhous when cultured with various carbon sources and soybean oil as co-substrates. Glucose was found to be the carbon source with best culture growth and astaxanthin production and the addition of 2% (v/v) soybean oil resulted in even higher astaxanthin producing. In addition, four carotenogenic genes encoding geranylgeranyl diphosphate synthase (crtE), phytoene desaturase (crtl), phytoene synthase lycopene cyclase(crtYB), and astaxanthin synthetase (ast), respectively, were demonstrated to be associated with different transcription levels under various substrates. The present study suggests the effectiveness of manipulating the metabolic regulation by using different carbon sources, in order to improve the production of astaxanthin.
Key words: Astaxanthin, carbon source, carotenogenic genes, Xanthophyllomyces dendrorhous, , ,
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Cite this Reference:
Wei Wu and Xin Yu, . Effect of Different Carbon Source on Expression of Carotenogenic Genes and Astaxanthin Production in Xanthophyllomyces dendrorhous. Advance Journal of Food Science and Technology, (10): 1375-1379.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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