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Abstract
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Article Information:
Purification and Characterization of a Bacteriocin Produced by Lactobacillus lactis Isolated from Marine Environment
G. Rajaram, P. Manivasagan, B. Thilagavathi, A. Saravanakumar
Corresponding Author: Manivasagan
Submitted: 2009 December, 30
Accepted: 2010 January, 16
Published: 2010 March, 10 |
Abstract:
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Bacteriocin producing Lactobacillus lactis strain isolated from marine environment, showed broad
range of antibacterial activity against some major food borne pathogens. Maximum bacteriocin production was
observed at 30°C , pH 6.0 and 1.5% sodium chloride solution. In addition of enzymes, "α-amylase, DNase,
RNase and lipase were slightly positive effect bacteriocin production. Proteinase K and pepsin were strongly
inhibited bacteriocin production. Among detergents, Sodium dodecyl sulphate (SDS), Tween 80 and Tritone
X-100 stimulated bacteriocin production and strongly inhibited by EDTA and urea. The bacteriocin has purified
by ammonium sulphate precipitate and ion exchange (DEAE cellulose) chromatography. Biochemically it was
pure protein moiety and the molecular weight was 94 kDA. The study revealed the possibility of using
bacteriocin as a food preservative and the L. lactis strain as probiotic.
Key words: Antagonistic activity, bacteriocin, indicator organisms, lactic acid bacteria, Lactobacillus lactis, ,
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Cite this Reference:
G. Rajaram, P. Manivasagan, B. Thilagavathi, A. Saravanakumar, . Purification and Characterization of a Bacteriocin Produced by Lactobacillus lactis Isolated from Marine Environment. Advance Journal of Food Science and Technology, (2): Page No: 138-144.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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