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2013 (Vol. 5, Issue: 12)
Article Information:

Application of Response Surface Methodology to Optimize Hydrolysis of Collagen from Croaker Scale

Y. Hong, W.W. Chen, Z.B. Jia and G.R. Huang
Corresponding Author:  W.W. Chen 

Key words:  Central composite rotatable design, degree of hydrolysis, response surface methodology, scale collagen, , ,
Vol. 5 , (12): 1646-1651
Submitted Accepted Published
September 04, 2013 September 17, 2013 December 05, 2013

Response Surface Methodology (RSM) based on a Central Composite Rotatable Design (CCRD) was applied to optimize the hydrolysis of collagen from croaker scale with the aim to maximize the Degree of Hydrolysis (DH). Effects of pH, temperature, the ratio of Enzyme to Substrate (E/S) and hydrolyzing time on DH were investigated. The results showed that the DH was predicted very well by a second-order polynomial model (R2 = 0.9153, p<0.0001). The highest DH was theoretically predicted to be 45.43%, under the optimal conditions (pH 8.0, 54.0 of temperature, 3.0% of E/S and 2.3 h of hydrolysis time). The recovery experimental DH under optimal conditions was calculated to be 46.63%, which confirmed the reliability of the method.
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  Cite this Reference:
Y. Hong, W.W. Chen, Z.B. Jia and G.R. Huang, 2013. Application of Response Surface Methodology to Optimize Hydrolysis of Collagen from Croaker Scale.  Advance Journal of Food Science and Technology, 5(12): 1646-1651.
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ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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