Abstract
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Article Information:
Application of TLC in the Screening of Acarbose-producing Actinomycetes
Fei Ren, Long Chen and Qunyi Tong
Corresponding Author: Qunyi Tong
Submitted: January 20, 2014
Accepted: January 30, 2014
Published: May 10, 2014 |
Abstract:
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Acarbose is widely used in medicine, such as the treatment of diabetes and obesity. A simple Thin Layer Chromatography (TLC)-scanning technique was developed for the rapid and accurate analysis of acarbose in a large number of fermentation broths of actinomycetes to screen for acarbose producer. The linearity of the acarbose in this way was good within the range from 2 to 10 μg (r2 = 0.9997). This technique didn’t need expensive instrument and complex procedure for the detection of acarbose in the fermentation broths. An actinomycete with acarbose yield of 1.83 mg/mL was obtained. The results demonstrated that TLC-scanning was a cheap and simple technique for the accurate screening of acarbose-producing actinomycetes.
Key words: Acarbose, actinomycetes, diabetes mellitus, screening, TLC, ,
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Abstract
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Cite this Reference:
Fei Ren, Long Chen and Qunyi Tong, . Application of TLC in the Screening of Acarbose-producing Actinomycetes. Advance Journal of Food Science and Technology, (5): 629-633.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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