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Abstract
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Article Information:
Mutation Breeding of Salt-tolerant and Ethanol-producing Strain S. cerevisiae H058 by Low-energy Ion Implantation
Shoubao Yan, Shunchang Wang, Zhijun Zhai, Xiangsong Chen and Jinyong Wu
Corresponding Author: Shoubao Yan
Submitted: May 19, 2014
Accepted: June 18, 2014
Published: July 10, 2014 |
Abstract:
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To obtain an industrial strain with high ethanol fermentation efficiency under salted conditions, the wild strain H058 of Saccharomyces cerevisiae was mutated by means of nitrogen ions implantation. Mutagenic effects of strain H058 by low energy N+ ion implantation were studied. A similar “saddle shape” survival curve due to ion beam irradiation appeared again in this study. By repeated screening, a high salt-tolerant and ethanol-producing strain M158 was obtained. Results showed that in medium contained 0, 1.5, 3.0, 4.5, 6.0% NaCl, M158 produced maximal ethanol of 98.3, 97.2, 96.4, 95.6 and 78.3 g/L at 54, 54, 54 and 72 h, respectively. However, the original strain H058 maximal ethanol of 95.2, 90.9, 84.8, 79.4 and 67.5 g/L at 60, 60, 66 and 72 h, respectively. In addition, the ethanol yield (g/g) in all of the NaCl concentrations for M158 is 0.492, 0.486, 0.482, 0.48 and 0.392 g/g, respectively, which were higher than those (0.476, 0.455, 0.424, 0.397 and 0.338 g/g, respectively) of the original strain H058. The higher production and shorter fermentation period suggest that strain M158 is a good salt-tolerant and ethanol-producing strain.
Key words: Breeding, ethanol fermentation, ion implantation, salt-tolerant, , , ,
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Cite this Reference:
Shoubao Yan, Shunchang Wang, Zhijun Zhai, Xiangsong Chen and Jinyong Wu, . Mutation Breeding of Salt-tolerant and Ethanol-producing Strain S. cerevisiae H058 by Low-energy Ion Implantation. Advance Journal of Food Science and Technology, (7): 941-946.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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