Abstract
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Article Information:
Optimized Ultrasonic-assisted Extraction of Flavonoids from Pyracantha fortuneana Fruit and Evaluation of Antibacterial Activities
Yun Zhang, Wei Wu, Xin Yu, Anju Xiao and Jing Lin
Corresponding Author: Wei Wu
Submitted: June 11, 2014
Accepted: July 19, 2014
Published: December 10, 2014 |
Abstract:
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Ultrasonic-Assisted Extraction (UAE) method was developed for extraction of total flavonoids from Pyracantha fortuneana fruit. The UAE parameters and the antioxidant activities of flavonoids extract were investigated. Important variables and their levels were obtained using single factor analysis method and central composite design. Through Response Surface Methodology (RSM) design experiments, the processing conditions were optimized as follows: ethanol concentration, 81.15%; liquid-solid ratio, 30.00 mL/g; extraction time, 3.14 h; and temperature, 69.55°C. Under the optimum condition, the extraction yield was 1.261%, which was well matched with the predicted values of 1.263%. The antibacterial activities of extract against four bacterial strains were determined by agar well diffusion method and the results indicated that the extract showed potent antibacterial activities, which concludes its application as natural antibacterial agent.
Key words: Antibacterial activities, central composite design, flavonoids, Pyracantha fortuneana, response surface methodology, ,
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Cite this Reference:
Yun Zhang, Wei Wu, Xin Yu, Anju Xiao and Jing Lin, . Optimized Ultrasonic-assisted Extraction of Flavonoids from Pyracantha fortuneana Fruit and Evaluation of Antibacterial Activities . Advance Journal of Food Science and Technology, (12): 1301-1306.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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