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Abstract
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Article Information:
The Development of an Electrochemical Immunosensory for 1-naphthylacetic Acid
S.Z. Liang, J.R. Pan, X.M. Yin and K. Sui
Corresponding Author: J.R. Pan
Submitted: March 17, 2015
Accepted: March 24, 2015
Published: August 25, 2015 |
Abstract:
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A new detection method for 1-naphthylacetic acid (NAA) based on the electrochemical immunosensor was introduced in the study, in which anti-NAA polyclonal antibody was used. An monolayer-molecular, self-reassemble membrane was made from 3-mercaptopropionic acid on the surface of gold electrodes, on the membrane the anti-NAA antibody was immoblize through ester-activated method with addition of 1-(3-Dimethylaminopropyl)-3- ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS). Based on the three-electrode system, an NAA immunosenory detection method was established with using the signal from cyclic voltammetry. The standard curve could be represented as △E = 0.0407lgc+0.0701(R2= 0.9984) with the range from 0.05 μg/mL to 2 &mug/mL and with the detection limit was 0.035 &mug/mL. The sensory method had very low cross-reactivity, lower than 3%, to NAA similarities such as phenylacetic acid and indolyl-3-acetic acid. In addition, the sensor could be used with high stability as long as 30 days (room temperature). It indicated that a specific, stable electrochemical immunosensory detection method for 1-Naphthaleneacetic acid was successfully developed.
Key words: 1-naphthylacetic acid, electrochemical immunosensor, self-assembled monolayer membrance, , , ,
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Cite this Reference:
S.Z. Liang, J.R. Pan, X.M. Yin and K. Sui, . The Development of an Electrochemical Immunosensory for 1-naphthylacetic Acid. Advance Journal of Food Science and Technology, (6): 439-443.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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