Home           Contact us           FAQs           
 
   Journal Page   |   Aims & Scope   |   Author Guideline   |   Editorial Board   |   Search
    Abstract
2011 (Vol. 3, Issue: 2)
Article Information:

The Possible Biochemical Mechanism of Action of Petroleum-Ether Extract of Calotropis procera Leaves (Asclepiadaceae) - A Potent Abortifacient in Gravid Dawley Rats

A.B. James, G.M. Saibu, O.A. Magbagbeola, A.K. Oloyo and B.A. Iwalokun
Corresponding Author:  Iwalokun Bamidele 

Key words:  Abortifacient, β-adrenergic receptor, Calotropis procera, cAMP, muscarinic receptor, Myosin Light Chain Kinase (MLCK), Protein Kinase A (PKA), uterine contraction
Vol. 3 , (2): 61-66
Submitted Accepted Published
2010 September, 21 2010 October, 27 2011 February, 25
Abstract:

Calotropis procera (Asclepiadaceae) is one of the traditionally used antifertility plants in Nigeria. Previous studies have shown that this antifertility plant has abortifacient property but none of them has reported its possible biochemical mechanism of action. Organ bath experiments using cumulative doses of the extract on rat uterine rings in Dejalonís solution aerated with 95% O2 + 5% CO2 produced an increase in tension (% response) with an EC50 value of 0.1064 mg/mL. Blocking muscarinic receptors with 35 μg/mL atropine caused the dose-response curve to slightly shift to the right (EC50 shift = 0.1064-0.1242), with a significant (p<0.05) decrease in maximal tension (%response). However, the β2-adrenergic blocker (1 μM propanolol) caused a twofold shift in the dose-response curve (EC50 shift = 0.1064-0.2591). The extract also exhibited its effect on cAMP modulation of β2-adrenergic receptors by causing a significant (p<0.05) decrease in cAMP level after treatment of cultured uterine cells with the extract. In conclusion, this study suggests that pet-ether extract of Calotropis procera (Asclepiadaceae) may exerts its abortifacient effect by inducing myometrial contractions of the uterus by binding to β2-adrenergic receptors thereby causing a decrease in the level of cAMP. cAMP reduction reduces the activation of Protein Kinase A (PKA) thereby preventing PKA from inactivating Myosin Light Chain Kinase (MLCK).
Abstract PDF HTML
  Cite this Reference:
A.B. James, G.M. Saibu, O.A. Magbagbeola, A.K. Oloyo and B.A. Iwalokun, 2011. The Possible Biochemical Mechanism of Action of Petroleum-Ether Extract of Calotropis procera Leaves (Asclepiadaceae) - A Potent Abortifacient in Gravid Dawley Rats.  Asian Journal of Medical Sciences, 3(2): 61-66.
    Advertise with us
 
ISSN (Online):  2040-8773
ISSN (Print):   2040-8765
Submit Manuscript
   Current Information
   Sales & Services
   Contact Information
  Executive Managing Editor
  Email: admin@maxwellsci.com
  Publishing Editor
  Email: support@maxwellsci.com
  Account Manager
  Email: faisalm@maxwellsci.com
  Journal Editor
  Email: admin@maxwellsci.com
  Press Department
  Email: press@maxwellsci.com
Home  |  Contact us  |  About us  |  Privacy Policy
Copyright © 2009. MAXWELL Science Publication, a division of MAXWELLl Scientific Organization. All rights reserved