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2012 (Vol. 4, Issue: 1)
Article Information:

Methanolic Extract of Moringa oleifera Lam Roots is Not Testis-Friendly to Guinea Pigs

C.W. Paul and B.C. Didia
Corresponding Author:  C.W. Paul 

Key words:  Histarchitecture, methanolic, Moringa oleifera lam, , , ,
Vol. 4 , (1): 47-54
Submitted Accepted Published
2012 January, 19 2012 February, 09 2012 February, 25
Abstract:

The moringa tree is native to the southern foothills of the Himalayes, and possibly Africa and the middle East. The root contains an active anti-biotic principle, pterygo-spermin. The root bark contains two alkaloids viz. moringine and moringinine. The aim of the study was to investigate effect (s) of methanolic extract of Moringa oleifera lam root on Histo-archtechture of testis of guinea pigs. The objectives were; To determine if methanolic extracts of Moringa oleifera lam root has effect (s) on the histology of testis, of guinea pigs. To determine whether the effects are dose-dependent and/or time-dependent. To ascertain the possible mechanism (s) by which M. oleifera lam root extracts achieves its effects based on histologic findings on tissues. ‘LD50 ip’ of 223.61 mg/kg was determined using modified Lorke’s (1983) method. Twenty four (24) Boars were enrolled for the study. Acclimatized and randomly distributed into groups A-C and control. They were given daily intra-peritoneal injection of methanolic extract of Moringa oleifera lam root for three (3) weeks. Doses of 3.6, 4.6 and 7.0 mg/kg were given to groups A, B and C, respectively. Four pigs; one from each group were sacrificed on 8th, 22nd and 43rd days, respectively. Tissues collected were immediately prepared histologically for haematoxylene and eosin stain. The photomicrographs were observed under the microscope with magnifications of X400 and X200. Sections of testis from control group showed normal histological features. Histological sections of testis from this group A showed seminiferous tubules with few spermatids and spermatozoa. Histological section of testis from group B revealed numerous seminiferous tubules with most having primary and secondary spermatogonia, spermatids and few mature spermatozoa. Group C showed seminiferous tubules with few spermatids and spermatozoa. Histological sections of testis in reversal group showed normal histology comparable to the control group. High doses and prolonged exposure to the Methanolic extract of Moringa oleifera lam root resulted in a spectrum of effects in the seminiferous tubules; ranging from formation of few spermatids and spermatozoa to no formation of spermatozoa the height of sertoli cells was shortened. The interstitial cells of leydig were nomal in size suggesting that hypothalamus and pituitary glands are not spared. Therefore, the mechanism of action of this extract on the Boars may be due to global effects of the extract on the male reproductive system. Methanolic extract of Moringa oleifera lam root caused distortion of the histoarchitecture of the testis of guinea pigs. The effects of the extract increased with time and increased dose of extract administered.
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  Cite this Reference:
C.W. Paul and B.C. Didia, 2012. Methanolic Extract of Moringa oleifera Lam Roots is Not Testis-Friendly to Guinea Pigs.  Asian Journal of Medical Sciences, 4(1): 47-54.
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ISSN (Online):  2040-8773
ISSN (Print):   2040-8765
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