This study was undertaken to evaluate the modulatory role of honey administration on blood glucose levels in non-diabetic Wistar rats within time intervals. Animals were randomly divided into four groups (n = 5): Rats in group I were given 1mL of distilled water and served as the control. Rats in group II, group III and IV, were administered 50, 100, 200 mg/kg body weight of honey respectively. After administration blood samples were collected from the tail artery of the rats at interval of 1, 4, 8 and 16 h, respectively. The study showed that there was no significant change (p>0.05) on levels of blood glucose levels in the groups administered with 50, 100 and 200 mg/kg b w of honey when compared to control group. The findings from the present study showed that honey has a mild effect on glycaemic response and therefore, may be recommended as a source of carbohydrate and even sweetening agent in place of sugar to diabetic patients.