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     Asian Journal of Medical Sciences

    Abstract
2012(Vol.4, Issue:5)
Article Information:

Identification of Multidrug-Resistant Genes in Acinetobacter baumannii in Sulaimani City-Kurdistan Regional Government of Iraq

Aras A.K. Shali
Corresponding Author:  Aras A.K. Shali 
Submitted: August 09, 2012
Accepted: September 17, 2012
Published: October 25, 2012
Abstract:
Acinetobacter baumannii is an opportunistic pathogen responsible for hospital-acquired infections. A. baumannii epidemics described world-wide were caused by few genotypic clusters of strains. The occurrence of epidemics caused by multi-drug resistant strains assigned to novel genotypes have been reported over the last few years. Multiple drug-resistant strains of Acinetobacterhave created therapeutic problems worldwide. This study was conducted to determine the antimicrobial susceptibility patterns of blaOXA-type carbapenemases among isolates of Acinetobacter. Twenty one Acinetobacter isolates were identified at the species level and their susceptibilities to different antibiotics were determined using VitekĀ® 2 system. Resistant percentages for all isolates were recorded; highest resistant rate was against ampicillin (100%) while lowest rate was against imipenem (57.1%). The MICs of imipenem for the resistant isolates were ≥16. All isolates show multi drug resistance to different antibiotics used. The Isolates were then subjected to multiplex-PCR targeting blaOXA genes. All strains of A. baumannii possess a blaOXA-51-like gene. The co-existence of blaOXA-51-like and blaOXA-23-like were detected in 17 strains while in 4 strains blaOXA-51-like was the only presented gene. Detection of blaOXA-51-like can be used as a simple and reliable method to differentiate A. baumannii strains from other species.

Key words:  blaOXA-51-like Acinetobacter baumannii, Kurdistan Acinetobacter baumannii, multidrug resistant A. baumannii, , , ,
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Cite this Reference:
Aras A.K. Shali, . Identification of Multidrug-Resistant Genes in Acinetobacter baumannii in Sulaimani City-Kurdistan Regional Government of Iraq. Asian Journal of Medical Sciences, (5): 179-183.
ISSN (Online):  2040-8773
ISSN (Print):   2040-8765
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