This study was carried out to determine the seroreactivity of blood of children attending Kogi Diagnostic and Reference Hospital, Anyigba to HIV-1/2 peptides and to evaluate some of the children’s demographic variables that are associated with anti-HIV-1/2 antibody positivity. We consecutively selected and screened 100 male and female children (aged ≤16 years). Blood sample of each child was screened for HIV-1/2 antibodies using Chembio HIV-1/2 Stat-Pak® and Abbott Determine HIV-1/2® test kits. Only participants that were sero-reactive to the two test kits were identified as seropositive for HIV-1/2 antibodies. The results showed a prevalence rate of 10.0% (n = 100). Risk factors for HIV infection like age, gender, and tribal marks appeared not significantly associated with anti-HIV-1/2 antibody positivity among the children (P = 0.142, P = 0.463 and P = 0.812, respectively). Conclusively, there was no sufficient evidence that age, gender and tribal marks were associated with positivity of HIV-1/2 antibodies among the children studied. This study has been able to add to the information on prevalence of HIV-1/2 antibodies among children in Anyigba, Kogi State, Nigeria. Education of children on HIV and AIDS should continue.