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Abstract
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Article Information:
Antagonistic Potentials of Marine Sponge Associated Fungi Aspergillus clavatus MFD15
A. Manilal, B. Sabarathnam, G.S. Kiran, S. Sujith, C. Shakir and J. Selvin
Corresponding Author: Aseer Manilal
Submitted: 2010 July, 03
Accepted: 2010 August, 29
Published: 2010 August, 30 |
Abstract:
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The development of resistance to multiple drugs is a major problem in the treatment of these
infectious diseases. Multidrug Resistant Staphylococcus aureus (MRSA) and Candida sp, the major infectious
agents have been recently reported in quite a large number of studies. With more intensive studies for natural
therapies, marine-derived products have been a promising source for the discovery of novel bioactive
compounds. A total of 45 marine fungi were isolated from the two sponges F. cavernosa and D. nigra were
screened for antimicrobial activity against pathogenic bacteria and fungi. The novel basal media formulated
in the present study resulted in increased frequency of fungal isolates when compared to all other media used
in the present study. The cell free supernatant of fungi exhibiting the broad spectrum of activity was subjected
to chemical analysis using different chromatographic systems including TLC, Column and GC-MS. Of the 15
fungal strains, 20% (3 strains) showed potential antagonistic activity against a panel of clinical pathogens used
in the present study. Based on the antimicrobial activity of the isolates, Aspergillus clavatus MFD15 was
recorded as potent producer displaying 100% activity against the tested pathogenic organisms. The TLC of the
crude ethyl acetate extract produced 3 spots with Rf values of 0.20, 0.79 and 0.95, respectively. The active TLC
fraction was purified in column chromatography which yielded 50 fractions. The active column fractions were
combined and analyzed with FT-IR, UV-Vis and GC-MS. The chemical analysis of the active compound
envisaged the active compound to be a triazole, 1H-1,2,4 Triazole 3- carboxaldehyde 5- methyl. The triazolic
compound was bacteriostic for S. aureus and bactericidal for E. coli. The triazole treated fabric showed 50%
reduction in the growth of E. coli, S. aureus, and S. epidermidis. Thus the purified compound can find a place
in the database for the development of fabrics with antimicrobial properties. This is the first report that
envisaged the production of triazole antimicrobial compound from sponge associated marine fungi from the
Indian coast.
Key words: Antimicrobial compound, Aspergillus clavatus, marine fungi, marine sponge, multidrug resistant pathogens, ,
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Cite this Reference:
A. Manilal, B. Sabarathnam, G.S. Kiran, S. Sujith, C. Shakir and J. Selvin, . Antagonistic Potentials of Marine Sponge Associated Fungi Aspergillus clavatus MFD15. Asian Journal of Medical Sciences, (4): Page No: 195-200.
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ISSN (Online): 2040-8773
ISSN (Print): 2040-8765 |
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