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Abstract
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Article Information:
Molecular Changes in Maspin and Bax Genes in Nitrosamine Induced Hepatocarcinogenesis
Saad M. EL-Gendy, Hayat Ibrahim, Sayed Mahdy, H.F. Gomaa, Ahmed M. Rashad and Mohamed Hessien
Corresponding Author: Mohamed Hessien
Submitted: 2010 May, 20
Accepted: 2010 June, 04
Published: 2010 November, 10 |
Abstract:
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This study was designed to evaluate the potential protective antioxidant and antihepatocarcinogenic
effects of beetroots in rats adm inistered nitrosamine precursors (dibutylamine (DBA) and sodium nitrate). Also
to monitor the changes in maspin and bax genes in parallel with the progression of nitrosamine induced
oxidative stress. Rats were divided into four groups (n = 25 each). Group I was treated with DBA/NaNO3,
group II was treated with DBA/NaNO3+ beetroots, in group III rats were treated with beetroots alone and group
IV was control group. Markers of oxidative stress and the hepatocarcinogenesis marker A lpha-Feto Protein
(AFP) were estimated in serum after 3, 6, 9 and 12 months. Simultaneously, the DNA levels of both cancer
related genes maspin and Box were evaluated in liver. The data demonstrated that beetroots was able to
antagonize the DBA/NaNO3-induced oxidative stress, where the levels of nitric oxide (NO), lipid peroxidation
(LPO) and reduced glutathion (GSH) were improved in beetroots protected rats compared to the DBA/NaNO3-
treated rats. This was accompanied with reduction in the level of AFP. The DNA content of maspin gene in
precarcinogenated animals decreased in time dependant manner, whereas the level of bax DNA content was
increased in parallel with the oxidative stress progression. No significant changes were observed in DNA
content of both genes in beetroots-treated group and control group. Histologically, nitrosamine precursors
showed distinct alterations such as loss of lobular architecture, fibrosis and the nuclei of many hepatocytes
appeared malignant. The antioxidative/antihepatocarcinogenesis effect of beetroots was reflected on the
histopathological architecture of liver and hepatocytes.
Key words: Box, beetroots, hepatocarcinogenesis, maspin, oxidative stress, ,
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Abstract
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Cite this Reference:
Saad M. EL-Gendy, Hayat Ibrahim, Sayed Mahdy, H.F. Gomaa, Ahmed M. Rashad and Mohamed Hessien, . Molecular Changes in Maspin and Bax Genes in Nitrosamine Induced Hepatocarcinogenesis. Asian Journal of Medical Sciences, (5): 201-210.
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ISSN (Online): 2040-8773
ISSN (Print): 2040-8765 |
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