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Abstract
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Article Information:
Screening and Characterization of Lactic Acid Bacteria from Fermented Milk
B. Lavanya, S. Sowmiya, S. Balaji and B. Muthuvelan
Corresponding Author: B. Muthuvelan
Submitted: 2010 December, 25
Accepted: 2011 February, 02
Published: 2011 April, 25 |
Abstract:
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In this study, different strains were isolated from the fermented milk in Vellore and were subjected
to preliminary screening and 45 isolates were obtained and it was characterized and examined for the presence
of probiotic properties like cholesterol assimilation, exopolysaccride production and antibiotic resistance. The
cholesterol assimilation ranged from 28-83%, which is significantly highest and observed for the first time, and
exopolysaccride varied from 16-89%. Further, resistance to 8 commonly used antibiotics β-lactans (penicillin,
ampicilin), gram positive spectrum (vanomycin), broad spectrum (rifampin, trimethoprim) and aminogycosides
(kanamycin, streptomycin, and bacitracin) was assessed by disk diffusion method. Among the selected 45
strains, 20, 20, 60, 70, 90 and 100% were found to be exhibiting a significant degree of resistance to kanamycin,
trimetroprim, rifampicin, kanamycin, amphicilin and penicillin respectively. However, all strains were resistant
to penicillin and 90% were resistant to ampicillin. Usually all Lactobacillus and Bifidobacterium strains were
susceptible to β-lactum antibiotics but our isolates showed resistance which is contrary and new information
to the previous investigation. Based on the above characters 7 isolates were considered to be best for probiotic
applications. The strains thus obtained are under investigation for further studies.
Key words: Antibiotics resistance, cholesterol assimilation, exopolysaccharide production, probiotics, , ,
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Abstract
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Cite this Reference:
B. Lavanya, S. Sowmiya, S. Balaji and B. Muthuvelan, . Screening and Characterization of Lactic Acid Bacteria from Fermented Milk. British Journal of Dairy Sciences, (1): 5-10.
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ISSN (Online): 2044-2440
ISSN (Print): 2044-2432 |
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