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Abstract
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Article Information:
Clastogenicity Potential Screening of Pleurotus pulmonarius and Pleurotus ostreatus Metabolites as Potential Anticancer and Antileukaemic Agents Using Micronucleus Assay
E.O. Akanni, J.K. Oloke, V.O. Mabayoje and G.O. Saka
Corresponding Author: Akanni E. Olufemi
Submitted: May 20, 2010
Accepted: June 04, 2010
Published: |
Abstract:
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Development of anticancer agents that will selectively destroy cancer cells without injury to normal
cells has led to the discovery of novel immunotherapeutic agents such as Pleurotus pulmonarius and Pleurotus ostreatus metabolites. This study is to screen the agents of dreadful side effects of causing mutation after a
prolonged use. Clastogenicity potential of the novel anti-cancer and antileukaemic agents Pleurotus
pulmonarius and Pleurotus ostreatus metabolites was evaluated in this study. Wister rats were grouped into
four with the test groups inoculated intraperitoneally at doses 64 and 16 mg/kg as 12.8 and 3.2% of the LD50
into the high and low dose rat groups respectively with each metabolite in a separate experiment. The treated
rats were sacrificed after 24, 48 and 72 h post treatment. Cyclophosphamide (clastogen) was inoculated into
the positive control group at doses 112 and 28 mg/kg w hile saline was used for the negative control group. In
all the treatment groups, only the rats in the positive control group formed micronuclei in their bone marrow
cells. There was only an increase in the formation of normochromatic and polychromatic erythrocytes in rat
groups inoculated with Pleurotus ostreatus metabolites. There is no statistically significant difference (p>0.05)
between the 3 post treatment sacrificing periods. Similar result was also obtained for Pleurotus pulmonarius
group. The chromosomal damaging potential screening reveals that the Pleurotus ostreatus and Pleurotus pulmonarius metabolites are not clastogenic (genotoxic) that is, unlikely to cause cancer producing mutations,
but rather enhanced erythropoiesis. They could therefore be useful anticancer agents when the potential is fully
explored.
Key words: Clastogen, genotoxicity, normochromatic, polychromatic erythrocytes, , ,
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Abstract
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Cite this Reference:
E.O. Akanni, J.K. Oloke, V.O. Mabayoje and G.O. Saka, . Clastogenicity Potential Screening of Pleurotus pulmonarius and Pleurotus ostreatus Metabolites as Potential Anticancer and Antileukaemic Agents Using Micronucleus Assay. British Journal of Pharmacology and Toxicology, (2): 56-61.
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ISSN (Online): 2044-2467
ISSN (Print): 2044-2459 |
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