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    Abstract
2011 (Vol. 3, Issue: 4)
Article Information:

Development of a Polymerase Chain Reaction (PCR) Assay for the Detection of Philippine Isolates of the Penaeus monodon-type Baculovirus (MBV)

Christopher Marlowe A. Caipang, May Flor J. Sibonga, Mary Jane A. Amar and Jane S. Geduspan
Corresponding Author:  Christopher Marlowe Caipang 

Key words:  MBV, monodon baculovirus, PCR, Penaeus monodon, shrimp, ,
Vol. 3 , (4): 416-420
Submitted Accepted Published
2011 May, 27 2011 June, 28 2011 July, 15
Abstract:

Penaeus monodon-type baculovirus (MBV) is a DNA virus that infects postlarvae and early juveniles of shrimp, Penaeus monodon. Several variants of this virus occur through nucleotide analysis of its genomic DNA. In the present study, a one-step PCR method was developed for the detection of the Philippine isolates of MBV by designing PCR primers on the least conserved region of the Philippine MBV. Using genomic DNA of MBV-infected shrimp postlarvae, the PCR assay amplified a 193-bp PCR product. Its sensitivity was comparable to the published PCR assays. The strain-specific primers did not cross-react with other DNA viruses including White Spot Syndrome Virus (WSSV), Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and hepatopancreatic parvovirus (HPV). This PCR assay could be used for regular monitoring and surveillance of MBV in shrimp as well as tracing the movement of the Philippine MBV in shrimp farms in different geographic regions.
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  Cite this Reference:
Christopher Marlowe A. Caipang, May Flor J. Sibonga, Mary Jane A. Amar and Jane S. Geduspan, 2011. Development of a Polymerase Chain Reaction (PCR) Assay for the Detection of Philippine Isolates of the Penaeus monodon-type Baculovirus (MBV).  Current Research Journal of Biological Sciences, 3(4): 416-420.
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ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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