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Abstract
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Article Information:
The Effects of Different Concentrations and Combinations of Growth Regulators on the Callus Formation of Potato (Solanum tubrosum) Explants
Shahab-ud-din, I.N. Sultan, M.A. Kakar, A. Yousafzai, F.A. Sattar, F. Ahmmad, S.M. Ibrahim, M. Hassanullah and B. Arif
Corresponding Author: Shahab-ud-din
Submitted: 2011 June, 27
Accepted: 2011 August, 08
Published: 2011 September, 10 |
Abstract:
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The main objective of the present study was to investigate the effects of different concentrations of
plant growth regulators and their combinations on callus induction of potato (Solanum tuberosum L.). The
explants of potato tuber were cultured on Modified Murashige and Skoog medium which was supplemented
with different concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene Acetic Acid (NAA),
Benzyl Adenine (BA), 2,4-D in combinations with BA and NAA in combination with BA for callus induction.
The Concentration of sucrose was 3% W/V level and the pH of the media was adjusted to 5.7 before the
addition of agar 8% W/V. The explants were first dissected out aseptically and then inoculated to the media
(with various levels of hormones), then incubated at 27±2ºC in the culture room. Among the treatments 2, 4-D
at different concentrations produced different degree of callus but comparatively a massive amount of callus
was formed on MS medium supplemented with 2, 4-D alone at 3.0 mg/L. Also NAA and BA with different
concentrations produced considerable degrees of callus but the degree of callus was best at higher
concentrations of NAA and BA. 2, 4-D in combination with BA at 2.0 mg/L both produced considerable
amount of callus. In case of NAA in combination with BA the degree of callus formation was best at
concentration 1.0 mg/L each. So according to the above findings it was concluded that 2, 4-D is the best option
for induction of callus among the other hormones used in the study.
Key words: Callus, explants, plant growth regulators, potato, , ,
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Cite this Reference:
Shahab-ud-din, I.N. Sultan, M.A. Kakar, A. Yousafzai, F.A. Sattar, F. Ahmmad, S.M. Ibrahim, M. Hassanullah and B. Arif, . The Effects of Different Concentrations and Combinations of Growth Regulators on the Callus Formation of Potato (Solanum tubrosum) Explants. Current Research Journal of Biological Sciences, (5): 499-503.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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