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2011 (Vol. 3, Issue: 5)
Article Information:

The Usage of -glucan Extracted from Local Mushroom as Immunomodulator

Mouruj A. Alaubydi and Reema M. Abed
Corresponding Author:  Mouruj A. Alaubydi 

Key words:  β-glucan, exchange, HPLC, immunomodulator, infilteration, ionic, local mushroom
Vol. 3 , (5): 535-541
Submitted Accepted Published
2011 May, 21 2011 August, 05 2011 September, 10
Abstract:

The present study was conducted to investigate the active compound of local cultured mushroom (β-glucan), and studied it effect immunologically on some organs (liver, spleen, small intestine). The results of drying 1 kg of fresh mushroom was 80 g dried material, the carbohydrates (polysaccharides) were extracted from the dried powder of mushroom by ethanol (99%).The carbohydrate and protein concentration were 88, 1.12 mg/mL, respectively, then the purification method was done for the extracted material, which were separated in to three main peaks, the carbohydrate concentration measured which were 490, 405 and 315 mg/mL, respectively, while Lowry method was detected the protein concentration for these peaks which were 0.0038, 0.0032 and 0.0023 mg/mL, respectively. HPLC carried out to identify β-glucan for these peaks. The results showed that the first peak was close one to standard β-glucan. The three peaks were examined as immunomodulator for lab. Animals (mice) that were divided into three groups according to the peaks of ion exchange diagram plus group of control. The results of immunization method showed crossly enlargement of liver, splean and small intestine, for all experimental animals groups, and microscopically changes in the tissues structure for these organs, generally there were infiltration of mononuclear cells in all these organs especially for the first group.
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  Cite this Reference:
Mouruj A. Alaubydi and Reema M. Abed, 2011. The Usage of -glucan Extracted from Local Mushroom as Immunomodulator.  Current Research Journal of Biological Sciences, 3(5): 535-541.
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ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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