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Abstract
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Article Information:
Characterization and Selection of Lactobacilli with Probiotic Properties from Cow’s Raw Milk of “Bororo” Cattle Breeders in Dschang (Cameroon)
Zambou Ngoufack François, Sieladie Djomne Victor, Fonteh A Florence, Kaktcham Pierre Marie and Morsi El Soda
Corresponding Author: Zambou Ngoufack François
Submitted: 2011 October, 26
Accepted: 2011 December, 09
Published: 2012 March, 10 |
Abstract:
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This study aimed at isolating potential probiotic lactobacilli strains from cow’s raw milk collected
near the small cattle breeders in Dschang (Cameroon) and applying established in vitro tests to screen those
strains that could be used as novel probiotic strains in the food industry. Thirty four lactobacilli isolates
obtained from raw cow’s milk were characterized and selected according to their preliminary probiotic
properties including tolerance to acid and bile salts, ability to assimilate cholesterol, sensitivity to antibiotics
and antimicrobial activities. Among the several requirements, the acid tolerance as well as the bile salt tolerance
was strain dependent. Nine isolates exhibited the highest surviving percentage at pH 3 and 2. Six acid tolerant
isolates presented an excellent tolerance at 0.2 and 0.4 % bile salts. The acid tolerant and bile tolerant isolates
were sensitive to most of the antibiotics tested. Six acid and bile tolerant isolates (6M, 11M, 12M, 21M, 48M)
exhibited antimicrobial activity against Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa,
Escherichia coli. These isolates reduced between 20.35 and 45.21 mg of cholesterol per g (dry weight) of cells
in vitro. They were identified using API 50CH kits and repetitive genomic element-PCR (Rep-PCR)
fingerprinting as Lactobacillus plantarum. According to the obtained results, these Lactobacillus plantarum
strains (6M 11M, 12M, 21M and 48M) were found to be the most promising strains with preliminary probiotic
properties.
Key words: Antibiotic susceptibility, cholesterol assimilation, Lactobacillus plantarum, probiotic, Rep-PCR, ,
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Cite this Reference:
Zambou Ngoufack François, Sieladie Djomne Victor, Fonteh A Florence, Kaktcham Pierre Marie and Morsi El Soda, . Characterization and Selection of Lactobacilli with Probiotic Properties from Cow’s Raw Milk of “Bororo” Cattle Breeders in Dschang (Cameroon). Current Research Journal of Biological Sciences, (2): 143-152.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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