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Abstract
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Article Information:
Effects of Zataria multiflora Extract as Rumen Modifiers Using in vitro Gas Production Technique
Mohammad Salamat Azar, Ramin Salamat Doust-Nober, Naser Maheri Sis, Habib Aghdam Shahryar and Yahya Asadi
Corresponding Author: mohammad salamatazar
Submitted: September 02, 2011
Accepted: October 07, 2011
Published: July 10, 2012 |
Abstract:
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This experiment was to determine effects of tow doses (0 and 0.15 mL/30 mL buffered rumen fluid)
of Zataria multiflora extract on Soybean Meal (SBM) degradability were studied by in vitro gas producing
techniques. Gas production test with mixtures of filtered rumen liquid of three taleshi native male cattle rumen
in times of 2, 4, 6, 8, 12, 24, 48, 72 and 96 were performed. The gas volumes for soybean meal in different
incubation times were higher than that of Zataria multiflora extract (0.15 mL/30 mL buffered rumen fluid).
Chemical composition for dry matter, crude protein and Non-Fibrous Carbohydrate were 94.07, 40 and 29.36%,
respectively. The results showed that gas volume at 48 h incubation (for 200 mg dry samples), were 62.12 and
38.01 mL/200 mg DM for soybean meal (control) and Zataria multiflora extract (0.15 mL/30 mL buffered
rumen fluid), respectively. the gas production from soluble fraction (a), the gas production from insoluble
fraction (b), rate constant of gas production during incubation (c) and the potential gas production (a + b)
contents of soybean meal (control) were 4.42 (mL/200 mg DM), 67.1 (mL/200 mg DM), 0.113 (mL/h) and
71.52 (mL/200 mg DM) respectively, the gas production from soluble fraction (a), the gas production from
insoluble fraction (b), rate constant of gas production during incubation (c) and the potential gas production (a
+ b) contents for Zataria multiflora extract (0.15 mL/30 mL buffered rumen fluid) were 3.52 (mL/200 mg DM),
45.54 (mL/200 mg DM), 0.07 (mL/h) and 49.06 (mL/200 mg DM), respectively. Gas volume at 72 h incubation
(for 200 mg dry samples), of soybean meal and Zataria multiflora extract (0.15 mL/30 mL buffered rumen
fluid) were 62.91 and 41.62, respectively. Gas volume at 96 h incubation (for 200 mg dry samples), of soybean
meal and Zataria multiflora extract (0.15 mL/30 mL buffered rumen fluid) were 63.72 and 42.82, respectively.
Key words: Gas production technique, insoluble fraction, soluble fraction, soybean meal, taleshi native male cattle, Zataria multiflora extract,
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Cite this Reference:
Mohammad Salamat Azar, Ramin Salamat Doust-Nober, Naser Maheri Sis, Habib Aghdam Shahryar and Yahya Asadi, . Effects of Zataria multiflora Extract as Rumen Modifiers Using in vitro Gas Production Technique. Current Research Journal of Biological Sciences, (4): 350-354.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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