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2012 (Vol. 4, Issue: 4)
Article Information:

Micro-Propagation of Vanilla planifolia Using Enset (Ensete ventricosum (Welw, cheesman)) Starch as a Gelling Agent

Ayelign Mengesha, Biruk Ayenew, Elias Gebremariam and Tewodros Tadesse
Corresponding Author:  Ayelign Mengesha 

Key words:  Alternative gelling agent, bulla starch, cost reduction, in vitro propagation, vanilla, ,
Vol. 4 , (4): 519-525
Submitted Accepted Published
May 09, 2012 June 01, 2012 July 10, 2012

Vanilla planifolia micro-propagation efficiency was evaluated using enset starch (bulla) as an alternative gelling agent. Particularly, optimization of bulla concentration levels and assessment of costs saved using bulla were carried out in this study since in vitro media ingredient, especially agar, comprises of the highest cost as compared to others ingredients. The results revealed that shoot numbers and fresh weight were significantly improved in multiplication media gelled with bulla (8%). Although all tested gelling agents apparent 100% rooting, increased newly growth of shoots was obtained on media supplemented with bulla (8%) followed by bulla (10%) and agar. Substitution of agar with bulla (8%) could save about 72.5% of agar cost per a litter media. Generally, replacing agar with 6-10% bulla could decline the cost of agar about 50-72.5% per a litter media. However, bulla resulted in poor media clarity that cause problem in contamination detection and enhanced root proliferation during multiplication phase. Hence, bulla seems to be more preferable in rooting phase that needs short growth period and improved the plantlets growth. In the future, detail studies would be needed on characterization of bulla starch to assure the principal active ingredients depending upon source of cultivars and locations as well as to assess effects on different crops.
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  Cite this Reference:
Ayelign Mengesha, Biruk Ayenew, Elias Gebremariam and Tewodros Tadesse, 2012. Micro-Propagation of Vanilla planifolia Using Enset (Ensete ventricosum (Welw, cheesman)) Starch as a Gelling Agent.  Current Research Journal of Biological Sciences, 4(4): 519-525.
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ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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