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    Abstract
2012 (Vol. 4, Issue: 5)
Article Information:

Molecular Study of nifH1, nifH2, nifH3, nifU, nifV, VF Genes and Classical Approach Cared out to Identification of Azotobacter chrococcum from Soil

Adel Kamal Khider
Corresponding Author:  Adel Kamal Khider 

Key words:  Azotobacter, Azotobacter chrococcum, nif gene, nitrogen fixation, PCR, ,
Vol. 4 , (5): 570-577
Submitted Accepted Published
May 24, 2012 June 23, 2012 September 20, 2012
Abstract:

The present study aimed to compare classical approach with molecular based method for identification of Azotobacter chrococcum from soil samples. A. chrococcum was isolated from soil source in Erbil city, Iraq. They were cultivated under laboratory conditions using Nitrogen free Azotobacter specific medium. A. chrococcum was present in all soil samples. result shows that A. chrococcum were rod shape, motility occur through the use of peritrichous flagella, cysts-forming, positive to oxidase, catalase and tryptophanase test, unable to liquefy gelatin, with insoluble brown or brown-black pigmentation and darken with age. Utilized starch, sucrose, mannitol and moloanat, but not rhamnose. molecular method based on detection of nifgenes have been successfully applied to describe A. chrococcum isolated from soil. The PCR products for nifH1 1102bp, nifH2 246bp, nifH3 128bp, nifU 930bp, nifV 1146bp and VF gene 594bp were detected on gel electrophoresis, while no bands observed for negative control. The isolated bacteria considered Azotobacte chrococcum belonging to Genus Azotobacter.
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  Cite this Reference:
Adel Kamal Khider, 2012. Molecular Study of nifH1, nifH2, nifH3, nifU, nifV, VF Genes and Classical Approach Cared out to Identification of Azotobacter chrococcum from Soil.  Current Research Journal of Biological Sciences, 4(5): 570-577.
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ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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