Home           Contact us           FAQs           
     Journal Home     |     Aim & Scope    |    Author(s) Information      |     Editorial Board     |     MSP Download Statistics
2013 (Vol. 5, Issue: 3)
Article Information:

Mitochondrial DNA A10398G Mutation is not Associated with Breast Cancer Risk in a Sample of Iraqi Women

Haneen M. Ismaeel, Hayba Q. Younan and Rawaa A. Zahid
Corresponding Author:  Haneen M. Ismaeel 

Key words:  Benign, malignant, mtDNA, PCR, polymorphism, ,
Vol. 5 , (3): 126-129
Submitted Accepted Published
December 26, 2012 January 21, 2013 May 20, 2013

The aim of this study was to investigate if there is a relationship between mtDNA polymorphism (A10398G) and breast cancer in a sample of 59 Iraqi women. Breast cancer is the second most common diagnosed cause of cancer death in the developed countries and accounts for 23% of the total cancers. Different studies reported that breast cancer accounts for 14% of all cancer deaths in females. It is well documented that the different factors such as genetics and environment factors are involved in tumorigenesis. Mutations in the mitochondrial DNA D-loop region and somatic mutations are emerging as early genetic markers of cancer. Identification of such markers for breast cancer would prevent late detection and increase the chance of recovery and survival rate. In breast cancer different mtDNA alterations were reported. The A10398G mutation in NADH Dehyrogenase (ND3) a subunit of complex I of the Oxidative Phosphorylation process (OXPHOS) is perhaps one of the most studied mutations with conflicting reports of its association with breast cancer. Genomic DNA was extracted from 21 unrelated women with malignant tumors, 22 women with benign tumors and 16 healthy women blood donors. Subsequently, PCR amplification was performed using specific primers, PCR products were subjected to a suitable restriction enzyme. No genetic variants were identified in mtDNA among malignant tumoral group and controls while 9% of benign tumor cases exhibited the variant. Our finding indicated that A10398G polymorphism cannot be used as a biomarker for breast cancer detection in Iraqi women.
Abstract PDF HTML
  Cite this Reference:
Haneen M. Ismaeel, Hayba Q. Younan and Rawaa A. Zahid, 2013. Mitochondrial DNA A10398G Mutation is not Associated with Breast Cancer Risk in a Sample of Iraqi Women.  Current Research Journal of Biological Sciences, 5(3): 126-129.
    Advertise with us
ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
Submit Manuscript
   Current Information
   Sales & Services
Home  |  Contact us  |  About us  |  Privacy Policy
Copyright © 2015. MAXWELL Scientific Publication Corp., All rights reserved