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Abstract
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Article Information:
Cloning and Characterization of Multiple RNA Splicing Variants of LDH-C Gene in Human and Rat
Qinglian Zhang, Min Yang, Jiagui Jin, Qinghua He and Jinhu Ma
Corresponding Author: Qinghua He
Submitted: March 02, 2013
Accepted: March 27, 2013
Published: July 20, 2013 |
Abstract:
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The expression of LDH-C (Lactate dehydrogenase C) gene is restricted in mature germ cells; however multiple splice variants of LDH-C expressed in human cancers and yak normal testes were reported recently. In order to know if there are any LDH-C splice variants in human normal testes, we set out to clone the putative variants in human and rat. Four splicing variants in human testes, 1 splicing variant in human spermatozoa, 6 splicing variants in rat testes and 1 splicing variant in rat non-testes tissues (liver, heart and muscle) were cloned. The putative polypeptides encoded by these variants were compared with the full-length LDH-C protein, the results showed that these putative polypeptides were truncated LDH-C proteins or truncated LDH-C proteins with a few amino acid residues different at N or C terminal. This suggested that these variants are possibly not used for translation, but targets of nonsense-mediated mRNA decay. Western blotting did not detect any bands with similar molecular weight as the putative polypeptides. RT-PCR showed that the expression levels of the splicing variants were significant during development of rat testes. The results indicate that LDH-C was not silenced by transcriptional repression in non-mature germ cells, but significantly transcripted and alternatively spliced.
Key words: Alternative splicing, development, expression regulation, lactate dehydrogenase c, splicing variant, ,
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Cite this Reference:
Qinglian Zhang, Min Yang, Jiagui Jin, Qinghua He and Jinhu Ma, . Cloning and Characterization of Multiple RNA Splicing Variants of LDH-C Gene in Human and Rat. Current Research Journal of Biological Sciences, (4): 182-189.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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