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Abstract
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Article Information:
Genomic Island Location in Acinetobacter baumannii Strains by tRIP-PCR Technique
Suhadsaad, Alice K. Melconian, Ali H. Ad'hiah and Kumar Rajakumar
Corresponding Author: Suhadsaad
Submitted: August 01, 2013
Accepted: August 26, 2013
Published: November 20, 2013 |
Abstract:
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This study was performed to detect the presence of genomic islands which usually insert in the tRNA genes and other non-coding RNA genes, in this study eight strains of Acinetobacter baumannii (AYE, A457, A14, A424 A473, A92, ACICU, A25) were tested by used of tRIP-(tRNA site Interrogation for Pathogeni city islands, prophases and other GIs)-PCR method. The results of PCR and agarose gel electrophoresis for eight strains of two loci #7, #24 were: the results of #7 loci screening showed that all strains were positive except A. baumannii 457 strain was negative. While the results of #24 loci showed presence of foreign DNA in A. baumannii AYE, A457, A14, A424, A473, A92 except the results of (ACICU, A25) was positive.
Key words: Acinetobacter baumannii, genomic islands, tRIP-PCR, , , ,
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Cite this Reference:
Suhadsaad, Alice K. Melconian, Ali H. Ad'hiah and Kumar Rajakumar, . Genomic Island Location in Acinetobacter baumannii Strains by tRIP-PCR Technique. Current Research Journal of Biological Sciences, (6): 296-301.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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