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2010 (Vol. 2, Issue: 4)
Article Information:

Identification of Rapd Primers Linked to Leaf Rust Resistant Gene Lr51 Introgressed from Triticum speltoides to Common Wheat (Triticum aestivum L.)

Khaist Begum, Habib Ahmad, Sajid ul Ghafoor, Sahib Gul Afridi, Ikram Muhammad and Imtiaz Ahmed Khan
Corresponding Author:  Imtiaz Ahmed Khan 

Key words:  Leaf rust, Lr51, molecular marker, PCR, RAPD, Triticum aestivum L., Triticum speltoides
Vol. 2 , (4): Page No: 226-228
Submitted Accepted Published
2009 December, 29 2010 January, 23 2010 July, 20
Abstract:

Leaf rust is the most important disease of wheat all over the world. Geneticists and breeder are now extracting new leaf rust resistant genes from wild relatives of wheat. An important leaf rust resistant gene Lr51 has been transferred from Triticum speltoides to common wheat. Present research was carried out to develop Randomly Amplified Polymorphic DNA (RAPD) based marker for the leaf rust resistant gene Lr51. Out of seven RAPD primers used, one RAPD prime viz; GLA-09 was found which could be used reliably to keep track of Lr51 in wheat background. This primer (GLA-09) amplified a polymorphic DNA fragment of approximately 1100 bp in the wheat lines having the leaf rust resistant gene Lr51.
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  Cite this Reference:
Khaist Begum, Habib Ahmad, Sajid ul Ghafoor, Sahib Gul Afridi, Ikram Muhammad and Imtiaz Ahmed Khan, 2010. Identification of Rapd Primers Linked to Leaf Rust Resistant Gene Lr51 Introgressed from Triticum speltoides to Common Wheat (Triticum aestivum L.).  Current Research Journal of Biological Sciences, 2(4): Page No: 226-228.
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