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Abstract
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Article Information:
Physiochemical, Functional and Structural Characterization of Wheat Germin Using In silico Methods
Vinita Hooda
Corresponding Author: Vinita Hooda
Submitted: 2010 October, 22
Accepted: 2010 November, 18
Published: 2011 January, 15 |
Abstract:
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Wheat germin, an oxalate oxidase possesses high proteolytic and thermal stability but is poorly
characterized. Three dimensional structure of wheat germin is also not available at PDB. The present paper uses
bioinformatics approach to describe the physiochemical, functional and structural properties of wheat germin.
The three dimensional structure of wheat germin is predicted by homology modelling using the highly
homologous barley germin as template. The model was evaluated with PROCHECK, VERIFY 3D, WHAT IF,
ERRAT and ProSA programs. Model visualization and analysis was done with Swiss-PDB viewer. Nature of
active site and key features of the model responsible for stability of enzyme are also discussed.
Key words: Homology modelling, oxalate oxidase, properties, stability, structure, ,
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Abstract
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Cite this Reference:
Vinita Hooda, . Physiochemical, Functional and Structural Characterization of Wheat Germin Using In silico Methods. Current Research Journal of Biological Sciences, (1): 35-41.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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