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Abstract
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Article Information:
Ethanol Production from Sago Waste Using Saccharomyces cerevisiae Vits-M1
D. Subashini, J. Ejilane, A. Radha, M.A. Jayasri and K. Suthindhiran
Corresponding Author: K. Suthindhiran
Submitted: 2010 October, 19
Accepted: 2010 November, 22
Published: 2011 January, 15 |
Abstract:
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The present study deals with the biotechnological production of ethanol from sago waste materials.
As petroleum has become depleted, renewable energy production has started to gain attention all over the
world, including the production of ethanol from sago wastes. In our research we have standardized the
production of ethanol from sago wastes using Saccharomyces cerevisiae strain isolated from molasses. The
production of ethanol was carried out by means of simultaneous saccharification with acids, followed by
fermentation. The yeast strains were isolated from either batter or molasses and the taxonomy was studied by
phenotypic characters in comparison with the standard strain Saccharomyces cerevisiae MTCC 173. Among
the two isolated strains, S. cerevisiae VITS-M1 isolated from molasses showed better survival rate in different
sugars such as glucose, sucrose, maltose and galactose except lactose; it also showed better survival rate at high
ethanol concentration and at acidic pH. The saccharification process of sago liquid waste and solid waste was
standardized using hydrochloric acid and sulphuric acid under different treatments. The fermented product,
ethanol was distilled using laboratory model distillation unit and measured qualitatively using gas
chromatography in comparison with the standard analytical grade ethanol. The overall experimental data
indicates that the sago liquid waste yielded more ethanol by simultaneous saccharification with 0.3N HCl and
0.3N H2SO4 and fermentation with the S. cerevisiae VITS-M1 isolated from molasses.
Key words: Ethanol, fermentation, saccharification, Saccharomyces cerevisiae VITS-M1, sago wastes, ,
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Cite this Reference:
D. Subashini, J. Ejilane, A. Radha, M.A. Jayasri and K. Suthindhiran, . Ethanol Production from Sago Waste Using Saccharomyces cerevisiae Vits-M1. Current Research Journal of Biological Sciences, (1): 42-51.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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