The main aim of this study is to isolate and identify the amylase producing bacteria from the estuarine environment. The water and sediment samples were collected from the uppanar estuary and the potential strains of amylase production were screened in starch agar plates. Totally 12 strains were collected, among them Bacillus megaterium exhibited the larges zone. Hence these potential strains were used for further optimization, production and purification of amylase enzyme. Optimizations and enzyme estimation were done through various factors. Here the enzyme productions were maximum at 35ºC, pH 7.5 and 30 ppt salt concentration.