A descriptive survey design was utilized to examine the relationship between multiple roles and the psychosocial well-being among non-academic female staff of a public university in Ghana. Purposive and simple random sampling methods were employed to recruit one hundred married working mothers as participants for the study. Questionnaires were used to collect data, which was analyzed using descriptive and Pearson Correlation statistics. The findings indicated a statistically significant negative relationship between respondents’ psychosocial well-being and some childcare and household responsibilities. While romantic activities were inversely correlated with the psychosocial well-being of the participants, the relationships were not statistically significant. The findings reinforce the need for healthcare and other professionals, such as social workers to deepen their understanding of the effects of multiple roles on the psychosocial well-being of women who combine employment with parenthood and marriage.