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2012 (Vol. 4, Issue: 6)
Article Information:

Effect of L-(+)-Ergothioneine (EGT) on Freezability of Ram Semen

U.C. Ari, R. Kulaksiz, Y. Ozturkler, S. Yildiz and N.C. Lehimcioglu
Corresponding Author:  U.C. Ari 

Key words:  Cryopreservation, L-ergothionine, poor-freezability, semen, tushin ram, ,
Vol. 4 , (6): 378-383
Submitted Accepted Published
September 14, 2012 November 08, 2012 December 20, 2012

The aim of this study was to investigate freezability of ram semen extended with different L-(+)-Ergothioneine (EGT) doses. For this aim, semen from four ram were collected with artificial vagina (44C) and then pooled. Pooled semen was divided five aliquots and extended with skim milk based extender containing 0 mmol/L (EGT0: Control), 1 mmol/L (EGT1), 2 mmol/L (EGT2), 5 mmol/L (EGT5) and 10 mmol/L (EGT10) EGT, respectively. After equilibration (+5C/2 h), the extended aliquots of semen in straws were cryopreserved in Liquid Nitrogen (LN2) vapour (-120C/15 min) and stored in LN2 (-196C) until examination date. Totally two straws from 17 replications (trials) in each experimental group were thawed in water bath (37C/1 min) and percentages of progressive motility, sperm viability, abnormality, acrosome and membrane integrity were determined and statistically assessed with SPSS. In the result, it was determined that different doses of EGT did not affect freezability of ram semen when seventeen replications considered (p>0.05). However, when separated trials according to good (≥20% for motility in control groups; 8 in 17 replications) or poor (<20% for motility in control groups; 9 in 17 replications) freezability were statistically analyzed, beneficial effects of 10 mmol/L concentration of EGT on progressive motility and membrane integrity were determined in poor freezability trials, compared with control (p<0.05). In conclusion, addition of EGT in semen extenders may be considered to improve freezability of ram semen, if there is a situation of poor-freezability.
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  Cite this Reference:
U.C. Ari, R. Kulaksiz, Y. Ozturkler, S. Yildiz and N.C. Lehimcioglu, 2012. Effect of L-(+)-Ergothioneine (EGT) on Freezability of Ram Semen.  International Journal of Animal and Veterinary Advances, 4(6): 378-383.
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ISSN (Online):  2041-2908
ISSN (Print):   2041-2894
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