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Abstract
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Article Information:
Histomorphological Comparison of Proventriculus and Small Intestine of Heavy and Light Line Pre- and at Hatching
Osama Nassir Wali and Khalid K. Kadhim
Corresponding Author: Khalid K. Kadhim
Submitted: December 09, 2013
Accepted: December 24, 2013
Published: February 20, 2014 |
Abstract:
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Gross, histomorphometric and histochemical comprasion of proventriculus and small intestinal segments were determined in heavy and light line at last day’s incubation. Five embryos from each line were used at 16 day, 19 day and 21 day incubation. The weight of the proventriculus, yolk sac and weight in addition to the length of each separated straightened intestinal segments were recorded. Histological samples were stained with Harris Hematoxylin and Eosin, Masson’s Trichrome stain for histological structures, combined Alcian blue-PAS and Aldehyde Fuschin-Alcian blue stain for mucin classification. The thickness of wall layers of these organs were determined, measurement of villus height, villus surface area and height of epithelial mucosa of each intestinal segments (duodenum, jejunum and ileum) were recorded. The relative weight of yolk sac, proventriculus, jejunum and ileum were significantly higher in heavy line. The relative length of intestine was not constant between breeds. However, the heavy line had longer jejunum and ileum at 16 day and 21 days incubation. Histologically, the well-developed proventricular glands and epithelial folds appeared since 16 day incubation in both breeds. The thicknesses of these glands was greater in heavy line. The duodenal of heavy line had dominantly longer villi with higher villus surface area, for jejunam was restricted at 21 days only. Similar pattern was found for epithelial height and number of goblet cells in intestinal mucosa. The histochemical observations showed presence of neutral and acidic mucin in intestinal epithelium with most mucous cells were had sulphated mucin. Intestinal development patterns were similar for both lines but growth was more rapid in heavyline than the light line.
Key words: Goblet cell, heavy and light line, histochemical, histomorphometric, surface area, villi,
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Cite this Reference:
Osama Nassir Wali and Khalid K. Kadhim, . Histomorphological Comparison of Proventriculus and Small Intestine of Heavy and Light Line Pre- and at Hatching. International Journal of Animal and Veterinary Advances, (1): 40-47.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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