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Abstract
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Article Information:
Effect of Escherichia coli and Staphylococcus aureus on the In vitro Response of Peripheral Blood Mononuclear Cells Isolated from Holstein Dairy Cows had Ketosis or Milk Fever of Field Cases
H. Ohtsuka, N. Kanikawa, K. Kudo, F. Kyutoku, S. Kakinuma, M. Kohno, Y. Maeda and T. Tanabe
Corresponding Author: H. Ohtsuka
Submitted: May 01, 2013
Accepted: May 31, 2013
Published: August 20, 2014 |
Abstract:
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The expression of cytokines mRNA, including Interleukin (IL)-2, IL-4 and Interferon (IFN)-&gamma and lymphocyte proliferation stimulated in vitro with either Escherichia coli (E. coli) or Staphylococcus aureus (S.A.) by Peripheral Blood Mononuclear Cells (PBMC) obtained from Holstein dairy cows were examined after the onset of ketosis or milk fever of field cases during the lactation period. The cows were used 50 to 150 days after calving in this study and divided into three groups; seventeen cows with milk fever (MF Group, 94.9±8.6 days after calving), ten cows with ketosis (Ketosis Group, 96.3±10.2 days after calving) and twenty-seven cows with no clinical diseases (Control Group, 89.4±5.4 days after calving). PBMC were stimulated in vitro by soluble antigen of E. coli or S.A. together with recombinant IL-2. The numbers of the peripheral blood T cells and monocyte were lower in the Ketosis Group in comparison with those in the Control Group and a significant difference was found in the number of CD3+TcR1-N12+T cells. There was a significantly lower lymphocyte proliferation stimulated with E. coli and S.A. in the Ketosis Group compared to that in the Control Group. Expression of IL-2, IL-4 and IFN-&gamma mRNA in PBMC stimulated with S.A. in the Ketosis Group was significantly lower than that in the Control Group, but these cytokine expression showed no group differences when PBMC were stimulated with E. coli. The present study demonstrated the onset of ketosis during the lactation period decreases reactivity of PBMC at least when they were stimulated with S.A in vitro.
Key words: Cytokine, dairy cows, immune function, ketosis, milk fever, ,
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Cite this Reference:
H. Ohtsuka, N. Kanikawa, K. Kudo, F. Kyutoku, S. Kakinuma, M. Kohno, Y. Maeda and T. Tanabe, . Effect of Escherichia coli and Staphylococcus aureus on the In vitro Response of Peripheral Blood Mononuclear Cells Isolated from Holstein Dairy Cows had Ketosis or Milk Fever of Field Cases. International Journal of Animal and Veterinary Advances, (4): 116-121.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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