Abstract
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Article Information:
Optimization of Cellulase Production by Trichoderma reesei HY07 Using Response Surface Methodology
Guowei Shu, Hui Yang, He Chen and Shikai Wang
Corresponding Author: Guowei Shu
Submitted: November 24, 2012
Accepted: January 17, 2013
Published: May 28, 2013 |
Abstract:
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Response Surface Methodology (RSM) based on a three-level, three-variable Box and Behnken Factorial Design (BBFD) was used to evaluate the interactive effects of corn stalk: bran ratio, tween80 and temperature on the cellulase production by solid fermentation. The optimum conditions derived via RSM were: corn stalk: bran ratio 1.29:1, Tween80 11.05 &muL and temperature 31 °C for carboxymethyl cellulase (CMCase) and corn stalk: bran ratio 0.77:1, Tween80 12.54&muL and temperature 32 °C for Filter Paper Activity (FPA). The actual experimental yield was 406.42 U/g for CMCase and 93.62 U/g for FPA under optimum condition, which compared well to the maximum predicted value of 405.67 U/g and 91.29 U/g. The cellulase yield under optimal conditions was 1.45 fold for CMCase and 1.33 fold for FPA to the control.
Key words: Cellulose, carboxymethyl cellulose, FPA, response surface methodology, solid state fermentation, ,
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Cite this Reference:
Guowei Shu, Hui Yang, He Chen and Shikai Wang, . Optimization of Cellulase Production by Trichoderma reesei HY07 Using Response Surface Methodology. Research Journal of Applied Sciences, Engineering and Technology, (23): 5438-5442.
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ISSN (Online): 2040-7467
ISSN (Print): 2040-7459 |
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