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2013 (Vol. 6, Issue: 09)
Article Information:

Improved Accumulation and Tolerant to Heavy Metals Triggered by Over-Expressing AtGCS from Arabidopsis Thaliana in E. coli

Dali Liu, Zhigang An, Zijun Mao and Zhenqiang Lu
Corresponding Author:  Zhigang An and Zijun Mao 

Key words:  Accumulation, AtGCS, E. coli, heavy metals tolerance, GSH content, ,
Vol. 6 , (09): 1578-1581
Submitted Accepted Published
November 09, 2012 January 07, 2013 July 15, 2013
Abstract:

GSH is one of the most ubiquitous proteins known to provide protection against toxic heavy metals. A gene-AtGCS encoding Arabidopsis &gamma-glutamylcysteine synthetase was introduced into Escherichia coli (BL21) by over-expression of TrxA-AtGCS fusion protein and analyzed the tolerant capacity to heavy metal by transformed E. coli. As comparison, the strain over-expressing TrxA was selected as the control. The results showed that the growth of E. coli cells over-expressing TrxA-AtGCS was much better than the control cells expressing TrxA under 1 mM Cd2+, Zn2+ and Cu2+ heavy metals respectively. Meanwhile, the higher bioaccumulation of Cd2+, Zn2+ and Cu2+ and GSH content were observed in the strain over-expressing TrxA-AtGCS. It could be concluded that over-expression of AtGCS offered a promising heavy metals resistance of E. coli with superior heavy metals accumulation and GSH content.
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  Cite this Reference:
Dali Liu, Zhigang An, Zijun Mao and Zhenqiang Lu, 2013. Improved Accumulation and Tolerant to Heavy Metals Triggered by Over-Expressing AtGCS from Arabidopsis Thaliana in E. coli.  Research Journal of Applied Sciences, Engineering and Technology, 6(09): 1578-1581.
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ISSN (Online):  2040-7467
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