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Abstract
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Article Information:
Improved Accumulation and Tolerant to Heavy Metals Triggered by Over-Expressing AtGCS from Arabidopsis Thaliana in E. coli
Dali Liu, Zhigang An, Zijun Mao and Zhenqiang Lu
Corresponding Author: Zhigang An and Zijun Mao
Submitted: November 09, 2012
Accepted: January 07, 2013
Published: July 15, 2013 |
Abstract:
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GSH is one of the most ubiquitous proteins known to provide protection against toxic heavy metals. A gene-AtGCS encoding Arabidopsis &gamma-glutamylcysteine synthetase was introduced into Escherichia coli (BL21) by over-expression of TrxA-AtGCS fusion protein and analyzed the tolerant capacity to heavy metal by transformed E. coli. As comparison, the strain over-expressing TrxA was selected as the control. The results showed that the growth of E. coli cells over-expressing TrxA-AtGCS was much better than the control cells expressing TrxA under 1 mM Cd2+, Zn2+ and Cu2+ heavy metals respectively. Meanwhile, the higher bioaccumulation of Cd2+, Zn2+ and Cu2+ and GSH content were observed in the strain over-expressing TrxA-AtGCS. It could be concluded that over-expression of AtGCS offered a promising heavy metals resistance of E. coli with superior heavy metals accumulation and GSH content.
Key words: Accumulation, AtGCS, E. coli, heavy metals tolerance, GSH content, ,
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Cite this Reference:
Dali Liu, Zhigang An, Zijun Mao and Zhenqiang Lu, . Improved Accumulation and Tolerant to Heavy Metals Triggered by Over-Expressing AtGCS from Arabidopsis Thaliana in E. coli. Research Journal of Applied Sciences, Engineering and Technology, (09): 1578-1581.
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ISSN (Online): 2040-7467
ISSN (Print): 2040-7459 |
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