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    Abstract
2009 (Vol. 1, Issue: 2)
Article Information:

Evaluation and Screening of Nitrogen Source for L-asparaginase Production by Aspergillus terreus MTCC 1782 using Latin Square Design

G. Baskar and S. Renganathan
Corresponding Author:  G. Baskar 

Key words:  Bioprocess, latin square design, optimization, shake culture fermentation, , ,
Vol. 1 , (2): Page No: 55-58
Submitted Accepted Published
2009 Sep., 02 2009 Sept., 14
Abstract:

The manufacture of enzymes, like L-asparaginase with less adverse effects in the treatment of cancer is an important facet of pharmaceutical industry. Yeast and Filamentous fungi are commonly reported in scientific literature to produce L-asparaginase with less adverse effects than prokaryotic microorganisms. In the present study Latin square design was used to examine the effect of L-proline, L-glutamic acid and Sodium nitrate as nitrogen sources on production of extracellular L-asparaginase by Aspergillus terreus MTCC 1782 in order to obtain the best nitrogen source in shake culture fermentation. The experimental values of L-asparaginase activity were subjected to statistical analysis and statistical significance of the nitrogen sources was studied for the Latin-Square Design using Dataplot software. It was found that L-proline as a best nitrogen source for the production of L-asparaginase by Aspergillus terreus MTCC 1782 for maximum L-asparaginase activity.
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  Cite this Reference:
G. Baskar and S. Renganathan, 2009. Evaluation and Screening of Nitrogen Source for L-asparaginase Production by Aspergillus terreus MTCC 1782 using Latin Square Design.  Research Journal of Mathematics and Statistics, 1(2): Page No: 55-58.
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