Abstract

The aim of this study was to evaluate the strain Lactobacillus plantarum C8 as starter culture for soybean sourdough fermentation, in terms of rheological properties, phenolic content and antioxidant activity. After this, wheat breads were prepared with fermented (sourdough) and unfermented soybean flour (control) at three different ratios (4.0:1.0, 3.5:1.0 and 1.5:1.0). Soybean sourdough showed less consistency and increased viscosity compared with unfermented dough. The content of phenolic compounds and the antioxidant activity significantly (p<0.05) increased after lactic fermentation. The overall characteristics of breads depended on the amount of soybean sourdough or flour addition while the best results were obtained with the lower substitution level. In fact, higher specific loaf volume, less crumb hardness and chewiness and intensification in lightness with respect to control breads, were obtained. Besides, sourdough breads showed high phenolic content and antioxidant activity with respect to breads made with unfermented soybean flour. In addition, sourdough breads were widely accepted by the consumers. This study put in evidence that sourdough addition improved the physical and functional properties of traditional wheat bread.

Keywords:

Antioxidant activity, Lactobacillus plantarum, physical properties, sourdough, soybean flour,

References

1. AOAC, 1990. Official Methods of Analysis. 15th Edn., Association of Official Analytical Chemists Inc., Arlington, VA, Vol. I.
   
2. Arendt, E.K., L.A.M. Ryan and F. Dal Bello, 2007. Impact of sourdough on the texture of bread. Food Microbiol., 24(2): 165-174.
   CrossRef    PMid:17008161    Direct Link
3. Banu, I. and I. Aprodu, 2012. Studies concerning the use of Lactobacillus helveticus and Kluyveromyces marxianus for rye sourdough fermentation. Eur. Food Res. Technol., 234(5): 769-777.
   CrossRef    Direct Link
4. Bartkiene, E., G. Juodeikiene and D. Vidmantiene, 2012. Nutritional quality of fermented defatted soya and flaxseed flours and their effect on texture and sensory characteristics of wheat sourdough bread. Int. J. Food Sci. Nutr., 63(6): 722-729.
   CrossRef    PMid:22229277    Direct Link
5. Benzie, I.F.F. and J.J. Strain, 1996. The ferric reducing ability of plasma (FRAP) as a measure of "Antioxidant Power": The FRAP assay. Anal. Biochem., 239(1): 70-76.
   CrossRef    PMid:8660627    Direct Link
6. Clarke, C.I., T.J. Schober, P. Dockery, K. O'Sullivan and E.K. Arendt, 2004. Wheat sourdough fermentation: Effects of time and acidification on fundamental rheological properties. Cereal Chem., 81(3): 409-417.
   CrossRef    Direct Link
7. Coda, R., C.G. Rizzello, D. Pinto and M. Gobbetti, 2012. Selected lactic acid bacteria synthesize antioxidant peptides during sourdough fermentation of cereal flours. Appl. Environ. Microbiol., 78(4): 1087-1096.
   CrossRef    PMid:22156436 PMCid:PMC3273010    Direct Link
8. De Vuyst, L. and M. Vancanneyt, 2007. Biodiversity and identification of sourdough lactic acid bacteria. Food Microbiol., 24(2): 120-127.
   CrossRef    PMid:17008154    Direct Link
9. Dhingra, S. and S. Jood, 2004. Effect of flour blending on functional, baking and organoleptic characteristics of bread. Int. J. Food Sci. Tech., 39(2): 213-222.
   CrossRef    Direct Link
10. Ezekiel, R., N. Singh, S. Sharma and A. Kaur, 2013. Beneficial phytochemicals in potato-a review. Food Res. Int., 50(2): 487-496.
    CrossRef    Direct Link
11. Gerez, C.L., M.I. Torino, G. Rollán and G.F. De Valdez, 2009. Prevention of bread mould spoilage by using lactic acid bacteria with antifungal properties. Food Control, 20(2): 144-148.
    CrossRef    Direct Link
12. Gerez, C.L., M.I. Torino, M.D. Obregozo and G. Font de Valdez, 2010. A ready-to-use antifungal starter culture improves the shelf life of packaged bread. J. Food Protect., 73(4): 758-762.
    CrossRef    PMid:20377968    Direct Link
13. He, F.J. and J.Q. Chen, 2013. Consumption of soybean, soy foods, soy isoflavones and breast cancer incidence: Differences between Chinese women and women in Western countries and possible mechanisms. Food Sci. Hum. Wellness, 2(3-4): 146-161.
    CrossRef    Direct Link
14. Hébert, E.M., R.R. Raya, P. Tailliez and G.S. de Giori, 2000. Characterization of natural isolates of Lactobacillus strains to be used as starter cultures in dairy fermentation. Int. J. Food Microbiol., 59(1-2): 19-27.
    CrossRef    Direct Link
15. Hole, A.S., I. Rud, S. Grimmer, S. Sigl, J. Narvhus and S. Sahlstrøm, 2012. Improved bioavailability of dietary phenolic acids in whole grain barley and oat groat following fermentation with probiotic Lactobacillus acidophilus, Lactobacillus johnsonii, and Lactobacillus reuteri. J. Agr. Food Chem., 60(25): 6369-6375.
    CrossRef    PMid:22676388    Direct Link
16. Houben, A., H. Götz, M. Mitzscherling and T. Becker, 2010. Modification of the rheological behavior of amaranth (Amaranthus hypochondriacus) dough. J. Cereal Sci., 51(3): 350-356.
    CrossRef    Direct Link
17. Jambrec, D.J., M.V. Pestoric, Ð.B. Psodorov, M.B. Sakac, N.M. Nedeljkovic, A.I. Mandic and I.J. Sedej, 2011. Instrumental and sensory properties of buckwheat flour pasta. Food Feed Res., 38(2): 45-50.
    Direct Link
18. Katina, K., 2005. Sourdough: A Tool for the Improved Flavour, Texture and Shelf-life of Wheat Bread. VTT. Tech. Res. Cent. of Finland VTT Publications 569, pp: 13-41, 53-75.
    Direct Link
19. López-Guel, E.C., F. Lozano-Bautista, R. Mora-Escobedo, R.R. Farrera-Rebollo, J. Chanona-Pérez, G.F. Gutiérrez-López et al., 2012. Effect of soybean 7S protein fractions, obtained from germinated and nongerminated seeds, on dough rheological properties and bread quality. Food Bioprocess Tech., 5(1): 226-234.
    CrossRef    Direct Link
20. Mamhoud, A., L. Nionelli, T. Bouzaine, M. Hamdi, M. Gobbetti and C.G. Rizzello, 2016. Selection of lactic acid bacteria isolated from Tunisian cereals and exploitation of the use as starters for sourdough fermentation. Int. J. Food Microbiol., 225: 9-19.
    CrossRef    PMid:26974248    Direct Link
21. Marazza, J.A., M.A. Nazareno, G.S. de Giori and M.S. Garro, 2012. Enhancement of the antioxidant capacity of soymilk by fermentation with Lactobacillus rhamnosus. J. Funct. Foods, 4(3): 594-601.
    CrossRef    Direct Link
22. Mashayekh, M., M.R. Mahmoodi and M.H. Entezari, 2008. Effect of fortification of defatted soy flour on sensory and rheological properties of wheat bread. Int. J. Food Sci. Tech., 43(9): 1693-1698.
    CrossRef    Direct Link
23. Moroni, A.V., F. Dal Bello, E. Zannini and E.K. Arendt, 2011. Impact of sourdough on buckwheat flour, batter and bread: Biochemical, rheological and textural insights. J. Cereal Sci., 54(2): 195-202.
    CrossRef    Direct Link
24. Nionelli, L., N. Curri, J.A. Curiel, R. Di Cagno, E. Pontonio et al., 2014. Exploitation of Albanian wheat cultivars: Characterization of the flours and lactic acid bacteria microbiota, and selection of starters for sourdough fermentation. Food Microbiol., 44: 96-107.
    CrossRef    PMid:25084651    Direct Link
25. Ribotta, P.D., S.A. Arnulphi, A.E. León and M.C. A-ón, 2005. Effect of soybean addition on the rheological properties and breadmaking quality of wheat flour. J. Sci. Food Agr., 85(11): 1889-1896.
    CrossRef    Direct Link
26. Rizzello, C.G., R. Coda, F. Mazzacane, D. Minervini and M. Gobbetti, 2012. Micronized by-products from debranned durum wheat and sourdough fermentation enhanced the nutritional, textural and sensory features of bread. Food Res. Int., 46(1): 304-313.
    CrossRef    Direct Link
27. Rizzello, C.G., A. Lorusso, M. Montemurro and M. Gobbetti, 2016. Use of sourdough made with quinoa (Chenopodium quinoa) flour and autochthonous selected lactic acid bacteria for enhancing the nutritional, textural and sensory features of white bread. Food Microbiol., 56: 1-13.
    CrossRef    PMid:26919812    Direct Link
28. Taghdir, M., S.M. Mazloomi, N. Honar, M. Sepandi, M. Ashourpour and M. Salehi, 2016. Effect of soy flour on nutritional, physicochemical, and sensory characteristics of gluten-free bread. Food Sci Nutr., Doi:10.1002/fsn3.411. https://doi.org/10.1002/fsn3.411
    CrossRef    Direct Link
29. Weenen, H., 1998. Reactive intermediates and carbohydrate fragmentation in Maillard chemistry. Food Chem., 62(4): 393-401.
    CrossRef    Direct Link
30. Wronkowska, M. and M. Soral-Smietana, 2008. Buckwheat flour-a valuable component of gluten-free formulations. Pol. J. Food Nutr. Sci., 58(1): 59-63.
    Direct Link
31. Yezbick, G., J. Ahn-Jarvis, S.J. Schwartz and Y. Vodovotz, 2013. Physicochemical characterization and sensory analysis of yeast-leavened and sourdough soy breads. J. Food Sci., 78(10): C1487-C1494.
    CrossRef    PMid:24024975 PMCid:PMC3879787    Direct Link

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The authors have no competing interests.

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