Abstract

The extraction of cellulase from commercial enzyme preparation by ammonium sulfate precipitation and its enzymatic characterization were studied. The results were as follows: the conditions of precipitation was 60% saturation of ammonium sulfate, the recovery rate of carboxymethylcellulase (CMCase) and Filter Paper enzyme Activity (FPA) were 73.8 and 71.4%, respectively. The enzyme has optimal temperature of 55°C, optimal pH of 4.8, the ions of $Co^{2+}$, $Mn^{2+}$ in the buffer lowered the activity of CMCase, but the $Cu^{2+}$ in low concentration activated the CMCase. The ions of $Co^{2+}$, $Mn^{2+}$ and $Al^{2+}$ in the buffer lowered the activity of FPA, but the $Cu^{2+}$ and $K^+$ in low concentration activated the FPA; $K_m$ and $V_{max}$ of the enzyme were $2.22×10^{-3}g/mL$ and $1.11×10^4 U/h$ using Lineweaver-Burk graphic method, respectively.

Keywords:

Carboxymethylcellulase, cellulase, filter paper activity, enzymatic property, salting out,

References

Competing interests

The authors have no competing interests.

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